TY - JOUR
T1 - D-dimer test after standardized coagulation. A new assay for global evaluation of the unstimulated fibrinolytic system
AU - Seljeflot, I.
AU - Arnesen, H.
AU - Eritsland, J.
AU - Smith, P.
PY - 1993/9
Y1 - 1993/9
N2 - In spite of recently developed analyses for specific components of the fibrinolytic system, a need for global tests still exists. Presently, most global testing is performed either after unpleasent stimulation of the fibrinolytic system, or with time-consuming and imprecise tests. In the present study we have evaluated a D-dimer test (ELISA method) in serum after standardized coagulation in unstimulated individuals, and related the results to those of t-PA and PAI-I activities. 28 healthy individuals, 49 patients with coronary heart disease (CHD) and 12 hypertriglyceridemic individuals were tested in the fasting state between 08:00 and 10:00. In the healthy individuals an arbitrary lower reference value of 0.34 ug/ml was established, whereas the median value was 0.86 ug/ml. In the CHD and hypertriglyceridemic groups, the median values were 0.41 ug/ml and 0.18ug/ml, respectively. When the individuals were grouped according to their values to t-PA and PAI-I activities, it emerged that the D-dimer in serum was influenced mainly by PAI-I, but that high values of t-PA determined the global test in some individuals. A good correlation was found between the D-dimer test and both the t-PA (r=0.63) and PAI-I (r=-0.71) activities. We conclude that the presently used D-dimer test in serum is suitable for global testing of the fibrinolytic system. Its clinical relevance has to be evaluated in clinical trials.
AB - In spite of recently developed analyses for specific components of the fibrinolytic system, a need for global tests still exists. Presently, most global testing is performed either after unpleasent stimulation of the fibrinolytic system, or with time-consuming and imprecise tests. In the present study we have evaluated a D-dimer test (ELISA method) in serum after standardized coagulation in unstimulated individuals, and related the results to those of t-PA and PAI-I activities. 28 healthy individuals, 49 patients with coronary heart disease (CHD) and 12 hypertriglyceridemic individuals were tested in the fasting state between 08:00 and 10:00. In the healthy individuals an arbitrary lower reference value of 0.34 ug/ml was established, whereas the median value was 0.86 ug/ml. In the CHD and hypertriglyceridemic groups, the median values were 0.41 ug/ml and 0.18ug/ml, respectively. When the individuals were grouped according to their values to t-PA and PAI-I activities, it emerged that the D-dimer in serum was influenced mainly by PAI-I, but that high values of t-PA determined the global test in some individuals. A good correlation was found between the D-dimer test and both the t-PA (r=0.63) and PAI-I (r=-0.71) activities. We conclude that the presently used D-dimer test in serum is suitable for global testing of the fibrinolytic system. Its clinical relevance has to be evaluated in clinical trials.
UR - http://www.scopus.com/inward/record.url?scp=0027442775&partnerID=8YFLogxK
U2 - 10.1016/0268-9499(93)90145-L
DO - 10.1016/0268-9499(93)90145-L
M3 - Article
AN - SCOPUS:0027442775
SN - 1369-0191
VL - 7
SP - 303
EP - 307
JO - Fibrinolysis and Proteolysis
JF - Fibrinolysis and Proteolysis
IS - 5
ER -