Cytokine production by allergen (Der pI)-specific CD4+ T cell clones derived from a patient with severe atopic disease

Paola Parronchi, Roberto Manetti, Cecilia Simonelli, Francesco Santoni Rugiu, Marie Pierre Piccinni, Enrico Maggi, Sergio Romagnani

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Twenty-four T cell clones (TCC) specific for purified Dermatophagoides pteronyssinus group I allergen (Der pI) were established from the peripheral blood of a patient with severe atopic disease and assessed for cytokine production in response to stimulation with both phorbol 12-myristate 13-acetate and anti-CD3 monoclonal antibody. All Der pI-specific TCC produced high amounts of interleukin (IL)-4 in association with IL-3, IL-5 and granulocyte monocyte-colony stimulating factor (GM-CSF), whereas they produced variable amounts of IL-2 and virtually no interferon-γ. These data support the hypothesis that atopy is associated with preferential activation of type 2 T helper cells and suggest a deregulation in the function of the IL-3, IL-4, IL-5 and GM-CSF gene cluster in subjects with severe atopic disorders.

Original languageEnglish
Pages (from-to)186-189
Number of pages4
JournalInternational Journal of Clinical & Laboratory Research
Volume21
Issue number2-4
DOIs
StatePublished - Jun 1992
Externally publishedYes

Keywords

  • Atopy
  • Granulocyte monocyte-colony stimulating factor
  • Interferon-γ
  • Interleukin-2, -3, -4 and -5
  • T cell clones

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