Cytogenetic characterization of 20 lymphoblastoid lines derived from human individuals differing in bleomycin sensitivity

T. C. Hsu, Edward J. Shillitoe, Lorraine M. Cherry, Qi Lin, Stimson P. Schantz, Cynthia Furlong

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Forty lymphoblastoid (lymphoid) lines were established from 42 volunteer blood donors, including healthy individuals and patients with head and neck carcinomas. Each peripheral blood sample was split into two portions, one for the establishment of a lymphoid line and the other for short-term culture, which was used to estimate bleomycin sensitivity by cytogenetic procedures. Twenty lymphoid lines were selected at random to compare bleomycin sensitivity with data obtained from short-term lymphocyte cultures. In each set, bleomycin sensitivity of lymphoid cells was similar to that of the lymphocytes. The lymphoid lines, which can be propagated for an unlimited supply of relatively homogeneous cellular material, will be useful for a variety of future investigations.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume26
Issue number1
DOIs
StatePublished - Jan 1990
Externally publishedYes

Keywords

  • bleomycin sensitivity
  • chromosome breakage
  • human
  • lymphoid lines

Fingerprint

Dive into the research topics of 'Cytogenetic characterization of 20 lymphoblastoid lines derived from human individuals differing in bleomycin sensitivity'. Together they form a unique fingerprint.

Cite this