TY - JOUR
T1 - Cyclin D1 overexpression in multiple myeloma
T2 - A morphologic, immunohistochemical, and in situ hybridization study of 71 paraffin-embedded bone marrow biopsy specimens
AU - Athanasiou, Evangelia
AU - Kaloutsi, Vassiliki
AU - Kotoula, Vassiliki
AU - Hytiroglou, Prodromos
AU - Kostopoulos, Ioannis
AU - Zervas, Costas
AU - Kalogiannidis, Panagiotis
AU - Fassas, Athanasios
AU - Christakis, John I.
AU - Papadimitriou, Constantine S.
PY - 2001/10
Y1 - 2001/10
N2 - Cyclin D1 expression was evaluated by immunohistochemical analysis and biotin-labeled in situ hybridization (ISH) in a series of 71 decalcified, paraffin-embedded bone marrow biopsy specimens from patients with multiple myeloma (MM). Cyclin D1 messenger RNA (mRNA) overexpression was detected by ISH in 23 (32%) of 71 cases, whereas cyclin D1 protein was identified by immunohistochemical analysis in 17 (24%) of 71 specimens. All cases that were positive by immunohistochemical analysis also were positive by ISH. Statistically significant associations were found between cyclin D1 overexpression and grade of plasma cell differentiation and between cyclin D1 overexpression and extent of bone marrow infiltration. Our findings demonstrate the following: (1) ISH for cyclin D1 mRNA is a sensitive method for the evaluation of cyclin D1 overexpression in paraffin-embedded bone marrow biopsy specimens with MM. (2) ISH is more sensitive than immunohistochemical analysis in the assessment of cyclin D1 expression. (3) Cyclin D1 overexpression in MM is correlated positively with higher histologic grade and stage.
AB - Cyclin D1 expression was evaluated by immunohistochemical analysis and biotin-labeled in situ hybridization (ISH) in a series of 71 decalcified, paraffin-embedded bone marrow biopsy specimens from patients with multiple myeloma (MM). Cyclin D1 messenger RNA (mRNA) overexpression was detected by ISH in 23 (32%) of 71 cases, whereas cyclin D1 protein was identified by immunohistochemical analysis in 17 (24%) of 71 specimens. All cases that were positive by immunohistochemical analysis also were positive by ISH. Statistically significant associations were found between cyclin D1 overexpression and grade of plasma cell differentiation and between cyclin D1 overexpression and extent of bone marrow infiltration. Our findings demonstrate the following: (1) ISH for cyclin D1 mRNA is a sensitive method for the evaluation of cyclin D1 overexpression in paraffin-embedded bone marrow biopsy specimens with MM. (2) ISH is more sensitive than immunohistochemical analysis in the assessment of cyclin D1 expression. (3) Cyclin D1 overexpression in MM is correlated positively with higher histologic grade and stage.
KW - Cyclin D1
KW - Immunohistochemistry
KW - In situ hybridization
KW - Multiple myeloma
KW - Paraffin-embedded bone marrow biopsy specimens
UR - http://www.scopus.com/inward/record.url?scp=18344380811&partnerID=8YFLogxK
U2 - 10.1309/BVT4-YP41-LCV2-5GT0
DO - 10.1309/BVT4-YP41-LCV2-5GT0
M3 - Article
C2 - 11601138
AN - SCOPUS:18344380811
SN - 0002-9173
VL - 116
SP - 535
EP - 542
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 4
ER -