TY - JOUR
T1 - Cyclic AMP responses are suppressed in mammalian cells expressing the yeast low Km cAMP-phosphodiesterase gene
AU - Van Lockeren Campagne, Michiel M.
AU - Wu, Erica
AU - Fleischmann, Robert D.
AU - Gottesman, Michael M.
AU - Chason, Kevin W.
AU - Kessin, Richard H.
PY - 1990
Y1 - 1990
N2 - A genomic DNA fragment from Saccharomyces cereuisiae which contains the SRA5 (=PDE2) gene, coding for a low Km cAMP-phosphodiesterase, was transfected into Chinese hamster ovary cells. Clones carrying the cAMP-phosphodiesterase gene were capable of growth in the presence of cholera toxin, which slows the growth of untransfected cells by elevating their cAMP levels. The cholera toxin-resistant transfected cell lines expressed high levels of cAMP-phosphodiesterase mRNA and cAMP-phosphodiesterase activity. Basal intracellular cAMP levels were not significantly affected by the presence of the yeast cAMP-phosphodiesterase gene, but elevation of cAMP levels in response to cholera toxin or prostaglandin El was suppressed. Induction of the c AMP-responsive tyrosine aminotransferase promoter by cholera toxin was also blocked in cell lines carrying the yeast cAMP-phosphodiesterase gene. Cholera toxin-resistant transfected cell lines were sensitive to the growth inhibitory effects of N6,O2′-dibutyryladenosine 3′,5′-monophosphate, which can be used to bypass the effects of the yeast cAMP-phosphodiesterase.
AB - A genomic DNA fragment from Saccharomyces cereuisiae which contains the SRA5 (=PDE2) gene, coding for a low Km cAMP-phosphodiesterase, was transfected into Chinese hamster ovary cells. Clones carrying the cAMP-phosphodiesterase gene were capable of growth in the presence of cholera toxin, which slows the growth of untransfected cells by elevating their cAMP levels. The cholera toxin-resistant transfected cell lines expressed high levels of cAMP-phosphodiesterase mRNA and cAMP-phosphodiesterase activity. Basal intracellular cAMP levels were not significantly affected by the presence of the yeast cAMP-phosphodiesterase gene, but elevation of cAMP levels in response to cholera toxin or prostaglandin El was suppressed. Induction of the c AMP-responsive tyrosine aminotransferase promoter by cholera toxin was also blocked in cell lines carrying the yeast cAMP-phosphodiesterase gene. Cholera toxin-resistant transfected cell lines were sensitive to the growth inhibitory effects of N6,O2′-dibutyryladenosine 3′,5′-monophosphate, which can be used to bypass the effects of the yeast cAMP-phosphodiesterase.
UR - http://www.scopus.com/inward/record.url?scp=0025213357&partnerID=8YFLogxK
M3 - Article
C2 - 1690715
AN - SCOPUS:0025213357
SN - 0021-9258
VL - 265
SP - 5840
EP - 5846
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 10
ER -