Objective: To investigate the anti-MCP-1 expression effect of curcumin in kidney disease model and the possible mechanisms associated with histone acetylation. Methods: ADR nephropathy was induced in male SD rats by twice intravenous injections of ADR at day 0 (5 mg·kg-1) and 1 week later (2.5 mg·kg-1). Curcumin treatment was started at week 6. EP300 and MCP-1 mRNA transcription were assessed by real-time PCR. The acetylation levels of the histones H3 and H4 in the promoters of MCP-1 were detected by ChIP-qPCR. And mouse podocytes were cultured to investigate the effect of curcumin against ADR-induced injury. Results: In animal model, curcumin intervention attenuated renal glomerulosclerosis and partially decreased transcription of EP300 and MCP-1 (P < 0.05) mRNA. Also, curcumin prevented an increase of acetylation of MCP-1 promoters H3 and H4 in ADR-nephropathy. In vitro cultured podocytes, compared with the untreated ADR group, pretreatment with low concentration of curcumin preserved podocyte cytoskeleton. Curcumin significantly reduced EP300 and MCP-1 mRNA (P < 0.01). Besides, curcumin prevented ADR-induced MCP-1 promoter hyper-acetylation in ADR injured podocytes by ChIP-qPCR. Conclusion: Curcumin prevents kidney fibrosis and decreases MCP-1 expression through modification of histone acetylation, and could be used as a therapy for patients with kidney disease.
|Number of pages
|International Journal of Clinical and Experimental Medicine
|Published - 30 Jul 2016
- Renal fibrosis