Culturing keratinocytes and fibroblasts in a three-dimensional mesh results in epidermal differentiation and formation of a basal lamina-anchoring zone

Paul Contard, Ronnda L. Bartel, Lloydstone Jacobs, Jerome S. Perlish, E. Douglas MacDonald, Lionel Handler, Diana Cone, Raul Fleischmajer

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

The purpose of this study was to characterize an in vitro co-culture model in which fibroblasts grown in a three-dimensional nylon mesh were recombined with human keratinocytes. The cultures were kept for 3 and 5 weeks and then processed for electron microscopy. Keratinocytes showed reconstruction of an epidermis consisting of a basal layer with hemidesmosomes, a stratified epithelium with tonofilaments and desmosomes, a granular layer with keratinosomes and keratohyaline granules, and a transitional stratum corneum. Anchoring filaments, lamina densa, anchoring fibrils, bundles of elastin-associated microfibrils (diameters 10 nm) and fine collagen fibrils were formed. Collagen fibrils near the epidermis were much thinner than those in the lower levels. The present study shows that the dermal model containing metabolically active fibroblasts in their natural environment will support epidermal morphogenesis and differentiation including the formation of a basal lamina and anchoring zone.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalJournal of Investigative Dermatology
Volume100
Issue number1
DOIs
StatePublished - Jan 1993

Fingerprint

Dive into the research topics of 'Culturing keratinocytes and fibroblasts in a three-dimensional mesh results in epidermal differentiation and formation of a basal lamina-anchoring zone'. Together they form a unique fingerprint.

Cite this