TY - JOUR
T1 - Crystallization of restriction endonuclease SfiI in complex with DNA
AU - Viadiu, Hector
AU - Scheuring Vanamee, Éva
AU - Jacobson, Eric M.
AU - Schildkraut, Ira
AU - Aggarwal, Aneel K.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The SfiI endonuclease from Streptomyces fimbriatus (EC 3.1.21.4) is a tetrameric enzyme that binds simultaneously to two recognition sites and cleaves both sites concertedly. It serves as a good model system for studying both specificity and cooperative DNA binding. Crystals of the enzyme were obtained by the hanging-drop vapor-diffusion method in complex with a 21-mer oligonucleotide. The crystals are trigonal, with unit-cell parameters a = b = 85.7, c = 202.6 Å, and diffract to 2.6 Å, resolution on a rotating-anode X-ray generator. Preliminary X-ray analysis reveals the space group to be either P3121 or P3221. Interestingly, the crystals change to space group P6122, with unit-cell parameters a = b = 85.5, c = 419.6 Å, when the selenomethionyl (SeMet) derivative of the enzyme is co-crystallized with the same DNA. Phase information is currently being derived from this SeMet SfiI-DNA complex.
AB - The SfiI endonuclease from Streptomyces fimbriatus (EC 3.1.21.4) is a tetrameric enzyme that binds simultaneously to two recognition sites and cleaves both sites concertedly. It serves as a good model system for studying both specificity and cooperative DNA binding. Crystals of the enzyme were obtained by the hanging-drop vapor-diffusion method in complex with a 21-mer oligonucleotide. The crystals are trigonal, with unit-cell parameters a = b = 85.7, c = 202.6 Å, and diffract to 2.6 Å, resolution on a rotating-anode X-ray generator. Preliminary X-ray analysis reveals the space group to be either P3121 or P3221. Interestingly, the crystals change to space group P6122, with unit-cell parameters a = b = 85.5, c = 419.6 Å, when the selenomethionyl (SeMet) derivative of the enzyme is co-crystallized with the same DNA. Phase information is currently being derived from this SeMet SfiI-DNA complex.
UR - http://www.scopus.com/inward/record.url?scp=0043069623&partnerID=8YFLogxK
U2 - 10.1107/S0907444903011910
DO - 10.1107/S0907444903011910
M3 - Article
C2 - 12876363
AN - SCOPUS:0043069623
SN - 0907-4449
VL - 59
SP - 1493
EP - 1495
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 8
ER -