Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

Bernard Axelrod; Janusz Steczko; Wladek Minor; Vivian Stojanoff

doi:10.1002/pro.5560040620

Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

Bernard Axelrod, Janusz Steczko, Wladek Minor, Vivian Stojanoff

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5 Scopus citations

Abstract

Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average M_r, 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, V_m = 2.48 Å³ /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).

Original language	English
Pages (from-to)	1233-1235
Number of pages	3
Journal	Protein Science
Volume	4
Issue number	6
DOIs	https://doi.org/10.1002/pro.5560040620
State	Published - Jun 1995
Externally published	Yes

Keywords

L‐3
lipoxygenase
lipoxygenase crystals
protein crystals
soybean

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@article{e319563957cb4137b40aa15c2242e5a6,

title = "Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans",

abstract = "Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20\% polyethylene glycol (average Mr, 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐{\AA} spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 {\AA} and β = 95.7°. The calculated value of Matthews's constant, Vm = 2.48 {\AA}3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).",

keywords = "L‐3, lipoxygenase, lipoxygenase crystals, protein crystals, soybean",

author = "Bernard Axelrod and Janusz Steczko and Wladek Minor and Vivian Stojanoff",

year = "1995",

month = jun,

doi = "10.1002/pro.5560040620",

language = "English",

volume = "4",

pages = "1233--1235",

journal = "Protein Science",

issn = "0961-8368",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

AU - Axelrod, Bernard

AU - Steczko, Janusz

AU - Minor, Wladek

AU - Stojanoff, Vivian

PY - 1995/6

Y1 - 1995/6

N2 - Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average Mr, 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, Vm = 2.48 Å3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).

AB - Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average Mr, 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, Vm = 2.48 Å3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).

KW - L‐3

KW - lipoxygenase

KW - lipoxygenase crystals

KW - protein crystals

KW - soybean

UR - https://www.scopus.com/pages/publications/0029068955

U2 - 10.1002/pro.5560040620

DO - 10.1002/pro.5560040620

M3 - Article

C2 - 7549886

AN - SCOPUS:0029068955

SN - 0961-8368

VL - 4

SP - 1233

EP - 1235

JO - Protein Science

JF - Protein Science

IS - 6

ER -

Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

Abstract

Keywords

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