Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

Bernard Axelrod, Janusz Steczko, Wladek Minor, Vivian Stojanoff

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average Mr, 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, Vm = 2.48 Å3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).

Original languageEnglish
Pages (from-to)1233-1235
Number of pages3
JournalProtein Science
Volume4
Issue number6
DOIs
StatePublished - Jun 1995
Externally publishedYes

Keywords

  • L‐3
  • lipoxygenase
  • lipoxygenase crystals
  • protein crystals
  • soybean

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