Crystallization and preliminary X-ray analysis of restriction endonuclease BamHI-DNA complex

T. Strzelecka, M. Newman, L. F. Dorner, R. Knott, I. Schildkraut, A. K. Aggarwal

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Restriction endonuclease BamHI from Bacillus amyloliquefaciens has been co-crystallized with a 12 bp DNA fragment that encompasses its recognition site. The co-crystals diffract to at least 1.95 Å resolution and belong to space group P212121. The unit cell parameters are a = 108.8 Å, b = 81.9 Å, c = 68.8 Å consistent with one complex in the crystallographic asymmetric unit. The direction of the DNA appears to be along the b axis. In order to achieve end to end stacking of DNA, the complex must lie on the screw axis along b. A selfrotation function has determined the directions of the non-crystallographic 2-fold axes.

Original languageEnglish
Pages (from-to)430-432
Number of pages3
JournalJournal of Molecular Biology
Volume239
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • BamHI
  • Co-crystallization
  • DNA binding protein
  • Restriction endonuclease
  • X-ray analysis

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