@article{573890712a0b4509b1056942fc8c96e7,
title = "Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni",
abstract = "Phosphoglycerate kinase (PGK) is indispensable during glycolysis for anaerobic glucose degradation and energy generation. Here we present comprehensive structure analysis of two putative PGKs from Bacillus anthracis str. Sterne and Campylobacter jejuni in the context of their structural homologs. They are the first PGKs from pathogenic bacteria reported in the Protein Data Bank. The crystal structure of PGK from Bacillus anthracis str. Sterne (BaPGK) has been determined at 1.68 {\AA} while the structure of PGK from Campylobacter jejuni (CjPGK) has been determined at 2.14 {\AA} resolution. The proteins' monomers are composed of two domains, each containing a Rossmann fold, hinged together by a helix which can be used to adjust the relative position between two domains. It is also shown that apo-forms of both BaPGK and CjPGK adopt open conformations as compared to the substrate and ATP bound forms of PGK from other species.",
keywords = "Anthrax, Bacillus anthracis, Campylobacter jejuni, Carbohydrate degradation, Gastroenteritis, Glycolysis, Guillain-Barr{\'e} syndrome, PGK, Pathogenic organism, Phosphoglycerate kinase, Rossmann fold",
author = "Heping Zheng and Filippova, {Ekaterina V.} and Tkaczuk, {Karolina L.} and Piotr Dworzynski and Maksymilian Chruszcz and Porebski, {Przemyslaw J.} and Zdzislaw Wawrzak and Olena Onopriyenko and Marina Kudritska and Sarah Grimshaw and Alexei Savchenko and Anderson, {Wayne F.} and Wladek Minor",
note = "Funding Information: Acknowledgments The authors would like to thank David R. Cooper, Ivan G. Shabalin, Jing Hou and the members of the Center of Structural Genomics for Infectious Diseases for valuable comments and discussions. This research was funded with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200700058C. Use of the Advanced Photon Source was supported by the U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor for the support of this research program (Grant 085P1000817).",
year = "2012",
month = mar,
doi = "10.1007/s10969-012-9131-9",
language = "English",
volume = "13",
pages = "15--26",
journal = "Journal of Structural and Functional Genomics",
issn = "1345-711X",
publisher = "Springer Netherlands",
number = "1",
}