Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

Gabriel Waksman; Dorothea Kominos; Scott C. Robertson; Nalin Pant; David Baltimore; Raymond B. Birge; David Cowburn; Hidesaburo Hanafusa; Bruce J. Mayer; Michael Overduin; Marilyn D. Resh; Carlos B. Rios; Lauren Silverman; John Kuriyan

doi:10.1038/358646a0

Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

Gabriel Waksman, Dorothea Kominos, Scott C. Robertson, Nalin Pant, David Baltimore, Raymond B. Birge, David Cowburn, Hidesaburo Hanafusa, Bruce J. Mayer, Michael Overduin, Marilyn D. Resh, Carlos B. Rios, Lauren Silverman, John Kuriyan

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Abstract

Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 Å, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

Original language	English
Pages (from-to)	646-653
Number of pages	8
Journal	Nature
Volume	358
Issue number	6388
DOIs	https://doi.org/10.1038/358646a0
State	Published - 1992
Externally published	Yes

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Waksman, G., Kominos, D., Robertson, S. C., Pant, N., Baltimore, D., Birge, R. B., Cowburn, D., Hanafusa, H., Mayer, B. J., Overduin, M., Resh, M. D., Rios, C. B., Silverman, L., & Kuriyan, J. (1992). Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. Nature, 358(6388), 646-653. https://doi.org/10.1038/358646a0

@article{d7792a00669a4205bd8ac65a449a23d1,

title = "Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides",

abstract = "Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 {\AA}, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.",

author = "Gabriel Waksman and Dorothea Kominos and Robertson, {Scott C.} and Nalin Pant and David Baltimore and Birge, {Raymond B.} and David Cowburn and Hidesaburo Hanafusa and Mayer, {Bruce J.} and Michael Overduin and Resh, {Marilyn D.} and Rios, {Carlos B.} and Lauren Silverman and John Kuriyan",

year = "1992",

doi = "10.1038/358646a0",

language = "English",

volume = "358",

pages = "646--653",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "6388",

}

Waksman, G, Kominos, D, Robertson, SC, Pant, N, Baltimore, D, Birge, RB, Cowburn, D, Hanafusa, H, Mayer, BJ, Overduin, M, Resh, MD, Rios, CB, Silverman, L & Kuriyan, J 1992, 'Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides', Nature, vol. 358, no. 6388, pp. 646-653. https://doi.org/10.1038/358646a0

TY - JOUR

T1 - Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

AU - Waksman, Gabriel

AU - Kominos, Dorothea

AU - Robertson, Scott C.

AU - Pant, Nalin

AU - Baltimore, David

AU - Birge, Raymond B.

AU - Cowburn, David

AU - Hanafusa, Hidesaburo

AU - Mayer, Bruce J.

AU - Overduin, Michael

AU - Resh, Marilyn D.

AU - Rios, Carlos B.

AU - Silverman, Lauren

AU - Kuriyan, John

PY - 1992

Y1 - 1992

N2 - Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 Å, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

AB - Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 Å, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

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U2 - 10.1038/358646a0

DO - 10.1038/358646a0

M3 - Article

C2 - 1379696

AN - SCOPUS:0026698924

SN - 0028-0836

VL - 358

SP - 646

EP - 653

JO - Nature

JF - Nature

IS - 6388

ER -

Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

Abstract

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