TY - JOUR
T1 - Crystal structure of PU.1/IRF-4/DNA ternary complex
AU - Escalante, Carlos R.
AU - Brass, Abraham L.
AU - Pongubala, Jagan M.R.
AU - Shatova, Ella
AU - Shen, Leyi
AU - Singh, Harinder
AU - Aggarwal, Aneel K.
N1 - Funding Information:
We thank the staff at APS (SBC, beamline 19ID) and NSLS (beamline X25) for facilitating X-ray data collection. A.K.A. is supported by a grant from the NIH (R01 AI41706). H.S. is an Investigator of the HHMI.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - The Ets and IRF transcription factor families contain structurally divergent members, PU.1, Spi-B and IRF-4 (Pip), IRF-8 (ICSBP), respectively, which have evolved to cooperatively assemble on composite DNA elements and regulate gene expression in the immune system. Whereas PU.1 recruits IRF-4 or IRF-8 to DNA, it exhibits an anticooperative interaction with IRF-1 and IRF-2. We report here the structure of the ternary complex formed with the DNA binding domains of PU.1 and IRF-4 on a composite DNA element. The DNA in the complex contorts into an unusual S shape that juxtaposes PU.1 and IRF-4 for selective electrostatic and hydrophobic interactions across the central minor groove. Together, the protein-protein and protein-DNA interactions provide insights into the stereochemical basis of cooperativity and anti-cooperativity between Ets and IRF factors.
AB - The Ets and IRF transcription factor families contain structurally divergent members, PU.1, Spi-B and IRF-4 (Pip), IRF-8 (ICSBP), respectively, which have evolved to cooperatively assemble on composite DNA elements and regulate gene expression in the immune system. Whereas PU.1 recruits IRF-4 or IRF-8 to DNA, it exhibits an anticooperative interaction with IRF-1 and IRF-2. We report here the structure of the ternary complex formed with the DNA binding domains of PU.1 and IRF-4 on a composite DNA element. The DNA in the complex contorts into an unusual S shape that juxtaposes PU.1 and IRF-4 for selective electrostatic and hydrophobic interactions across the central minor groove. Together, the protein-protein and protein-DNA interactions provide insights into the stereochemical basis of cooperativity and anti-cooperativity between Ets and IRF factors.
UR - http://www.scopus.com/inward/record.url?scp=18744377964&partnerID=8YFLogxK
U2 - 10.1016/S1097-2765(02)00703-7
DO - 10.1016/S1097-2765(02)00703-7
M3 - Article
C2 - 12453417
AN - SCOPUS:18744377964
SN - 1097-2765
VL - 10
SP - 1097
EP - 1105
JO - Molecular Cell
JF - Molecular Cell
IS - 5
ER -