Cryoprotected Lactobacillus casei: An approach to standardization of microbiological assay of folic acid in serum

N. Grossowicz, S. Waxman, C. Schreiber

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at-18°C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microrganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay of various nutriments. Standard folate growth curves of cryoprotected L. casei, prepared at intervals over eight months, are superimposable, have a low blank, and should eliminate the variations encountered with the continuous passages of microorganisms required for the classic microbiological assays. Serum folate values obtained by use of the cryoprotected L. casei fall into the same diagnostic groups as determined by the classic microbiological assay.

Original languageEnglish
Pages (from-to)745-747
Number of pages3
JournalClinical Chemistry
Volume27
Issue number5
DOIs
StatePublished - 1981
Externally publishedYes

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