CRISPR activation screens: navigating technologies and applications

Teleri Clark; Matthew A. Waller; Lipin Loo; Cesar L. Moreno; Christopher E. Denes; G. Gregory Neely

doi:10.1016/j.tibtech.2024.02.007

CRISPR activation screens: navigating technologies and applications

Teleri Clark
, Matthew A. Waller
, Lipin Loo
, Cesar L. Moreno
, Christopher E. Denes
, G. Gregory Neely

Research output: Contribution to journal › Review article › peer-review

45 Scopus citations

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

Original language	English
Pages (from-to)	1017-1034
Number of pages	18
Journal	Trends in Biotechnology
Volume	42
Issue number	8
DOIs	https://doi.org/10.1016/j.tibtech.2024.02.007
State	Published - Aug 2024
Externally published	Yes

Keywords

CRISPR screens
CRISPRa
gene activation
genetic screening

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10.1016/j.tibtech.2024.02.007

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abstract = "Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.",

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AU - Loo, Lipin

AU - Moreno, Cesar L.

AU - Denes, Christopher E.

AU - Neely, G. Gregory

PY - 2024/8

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AB - Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

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JO - Trends in Biotechnology

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CRISPR activation screens: navigating technologies and applications

Abstract

Keywords

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