CRISPR activation screens: navigating technologies and applications

Teleri Clark; Matthew A. Waller; Lipin Loo; Cesar L. Moreno; Christopher E. Denes; G. Gregory Neely

doi:10.1016/j.tibtech.2024.02.007

CRISPR activation screens: navigating technologies and applications

Teleri Clark, Matthew A. Waller, Lipin Loo, Cesar L. Moreno, Christopher E. Denes, G. Gregory Neely

Research output: Contribution to journal › Review article › peer-review

4 Scopus citations

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

Original language	English
Pages (from-to)	1017-1034
Number of pages	18
Journal	Trends in Biotechnology
Volume	42
Issue number	8
DOIs	https://doi.org/10.1016/j.tibtech.2024.02.007
State	Published - Aug 2024
Externally published	Yes

Keywords

CRISPR screens
CRISPRa
gene activation
genetic screening

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abstract = "Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.",

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AU - Neely, G. Gregory

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AB - Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

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CRISPR activation screens: navigating technologies and applications

Abstract

Keywords

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