TY - JOUR
T1 - Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
AU - Keogh, Michael Christopher
AU - Kurdistani, Siavash K.
AU - Morris, Stephanie A.
AU - Ahn, Seong Hoon
AU - Podolny, Vladimir
AU - Collins, Sean R.
AU - Schuldiner, Maya
AU - Chin, Kayu
AU - Punna, Thanuja
AU - Thompson, Natalie J.
AU - Boone, Charles
AU - Emili, Andrew
AU - Weissman, Jonathan S.
AU - Hughes, Timothy R.
AU - Strahl, Brian D.
AU - Grunstein, Michael
AU - Greenblatt, Jack F.
AU - Buratowski, Stephen
AU - Krogan, Nevan J.
N1 - Funding Information:
We thank M. Kim and J. Ingles for helpful discussions. We are grateful to K. Struhl, P. Mason, F. Winston, S. Roth-Dent, R. Sternglatz, J. Parvin, E. O’Shea, and D. Moazed for supplying research materials. We thank J. Workman for communicating results prior to publication and N. Datta, H. Ding, K. Ghandi, X. Wu, G. Zhong, and X. Guo for technical assistance. N.J.K. was supported by a Doctoral Fellowship from the Canadian Institutes of Health Research (CIHR), S.A.M. was supported by a predoctoral Ford fellowship, and B.D.S. was supported by a Pew Scholarship in the Biomedical Sciences. This research was supported by grants to J.F.G. from the CIHR, Genome Canada through the Ontario Genomics Institute, and the National Cancer Institute of Canada with funds from the Canadian Cancer Society; to C.B. from CIHR and Genome Canada through the Ontario Genomics Institute; and to B.D.S., M.G., and S.B. from the US National Institutes of Health.
PY - 2005/11/18
Y1 - 2005/11/18
N2 - The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.
AB - The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.
UR - https://www.scopus.com/pages/publications/27744587302
U2 - 10.1016/j.cell.2005.10.025
DO - 10.1016/j.cell.2005.10.025
M3 - Article
C2 - 16286008
AN - SCOPUS:27744587302
SN - 0092-8674
VL - 123
SP - 593
EP - 605
JO - Cell
JF - Cell
IS - 4
ER -