Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2

Alexander Sartorius; Patrick Schloss; Barbara Vollmayr; Gabriele Ende; Claudia Neumann-Haefelin; Mathias Hoehn; Fritz A. Henn

doi:10.1080/15622970600946446

Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2

Alexander Sartorius, Patrick Schloss, Barbara Vollmayr, Gabriele Ende, Claudia Neumann-Haefelin, Mathias Hoehn, Fritz A. Henn

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Hippocampal choline-containing compounds (Cho) determined with ¹H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p=0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.

Original language	English
Pages (from-to)	246-250
Number of pages	5
Journal	World Journal of Biological Psychiatry
Volume	7
Issue number	4
DOIs	https://doi.org/10.1080/15622970600946446
State	Published - Nov 2006
Externally published	Yes

Keywords

Choline-containing compounds
ECT
Hippocampus
MRS
Phospholipase

Access to Document

10.1080/15622970600946446

Cite this

@article{82368d9e46074d64a260c83676e69605,

title = "Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2",

abstract = "Hippocampal choline-containing compounds (Cho) determined with 1H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p=0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.",

keywords = "Choline-containing compounds, ECT, Hippocampus, MRS, Phospholipase",

author = "Alexander Sartorius and Patrick Schloss and Barbara Vollmayr and Gabriele Ende and Claudia Neumann-Haefelin and Mathias Hoehn and Henn, {Fritz A.}",

note = "Funding Information: This work was supported by the Deutsche For-schungsgemeinschaft (SFB636) to P.S., G.E. and F.A.H. Part of these data have been presented at the 42nd Annual Meeting of the American College of",

year = "2006",

month = nov,

doi = "10.1080/15622970600946446",

language = "English",

volume = "7",

pages = "246--250",

journal = "World Journal of Biological Psychiatry",

issn = "1562-2975",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2

AU - Sartorius, Alexander

AU - Schloss, Patrick

AU - Vollmayr, Barbara

AU - Ende, Gabriele

AU - Neumann-Haefelin, Claudia

AU - Hoehn, Mathias

AU - Henn, Fritz A.

N1 - Funding Information: This work was supported by the Deutsche For-schungsgemeinschaft (SFB636) to P.S., G.E. and F.A.H. Part of these data have been presented at the 42nd Annual Meeting of the American College of

PY - 2006/11

Y1 - 2006/11

N2 - Hippocampal choline-containing compounds (Cho) determined with 1H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p=0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.

AB - Hippocampal choline-containing compounds (Cho) determined with 1H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p=0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.

KW - Choline-containing compounds

KW - ECT

KW - Hippocampus

KW - MRS

KW - Phospholipase

UR - http://www.scopus.com/inward/record.url?scp=33750445927&partnerID=8YFLogxK

U2 - 10.1080/15622970600946446

DO - 10.1080/15622970600946446

M3 - Article

C2 - 17071545

AN - SCOPUS:33750445927

SN - 1562-2975

VL - 7

SP - 246

EP - 250

JO - World Journal of Biological Psychiatry

JF - World Journal of Biological Psychiatry

IS - 4

ER -

Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2

Abstract

Keywords

Access to Document

Fingerprint

Cite this