Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming

Michael G. Daniel, Ihor R. Lemischka, Kateri Moore

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations

Abstract

Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), the inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Yamanaka informs our ability to convert cell fates and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF-based reprogramming strategies to convert several cell types into HSCs.

Original languageEnglish
Pages (from-to)24-35
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume1370
Issue number1
DOIs
StatePublished - 1 Apr 2016

Keywords

  • cell fate conversion
  • hematopoiesis
  • hematopoietic stem cells
  • reprogramming
  • transcription factors

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