Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: Effect on sperm velocity

J. M. Colon; P. Sarosi; P. G. McGovern; A. Askin; J. M. Dziedzic; J. Skurnick; G. Weiss; E. M. Bonder

doi:10.1016/S0015-0282(16)54926-7

Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: Effect on sperm velocity

J. M. Colon
, P. Sarosi
, P. G. McGovern
, A. Askin
, J. M. Dziedzic
, J. Skurnick
, G. Weiss
, E. M. Bonder

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Individual human sperm can be micromanipulated in three dimensions using a 1.06 μm Nd:YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 μm/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.

Original language	English
Pages (from-to)	695-698
Number of pages	4
Journal	Fertility and Sterility
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/S0015-0282(16)54926-7
State	Published - 1992
Externally published	Yes

Keywords

Laser optical trap
human sperm
sperm velocity

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10.1016/S0015-0282(16)54926-7

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title = "Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: Effect on sperm velocity",

abstract = "Individual human sperm can be micromanipulated in three dimensions using a 1.06 μm Nd:YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 μm/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.",

keywords = "Laser optical trap, human sperm, sperm velocity",

author = "Colon, \{J. M.\} and P. Sarosi and McGovern, \{P. G.\} and A. Askin and Dziedzic, \{J. M.\} and J. Skurnick and G. Weiss and Bonder, \{E. M.\}",

note = "Funding Information: Received June 6, 1991; revised and accepted November 13, 1991. * Supported in part by grant HD-24649 from the National Institutes of Health, Bethesda, Maryland. t Presented at the 38th Annual Meeting of the Society for Gynecologic Investigation, San Antonio, Texas, March 20 to 23, 1991. *D epartment of Obstetrics and Gynecology, University of Medicine and Dentistry-New Jersey Medical School. § Reprint requests: Jose M. Colon, M.D., Department of Obstetrics and Gynecology, University of Medicine and DentistryNew Jersey Medical School, MSB E-506, 185 South Orange Avenue, Newark, New Jersey 07103. II Present address: 88 University Place, New York, New York. If Electric engineer, Department of Laser Science Research, AT\&T Bell Laboratories. \# Department of Preventive Medicine, University of Medicine and Dentistry of New Jersey-New Jersey Medical School. ** Department of Biological Science, Rutgers State University of New Jersey.",

year = "1992",

doi = "10.1016/S0015-0282(16)54926-7",

language = "English",

volume = "57",

pages = "695--698",

journal = "Fertility and Sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

number = "3",

}

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T1 - Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap

T2 - Effect on sperm velocity

AU - Colon, J. M.

AU - Sarosi, P.

AU - McGovern, P. G.

AU - Askin, A.

AU - Dziedzic, J. M.

AU - Skurnick, J.

AU - Weiss, G.

AU - Bonder, E. M.

N1 - Funding Information: Received June 6, 1991; revised and accepted November 13, 1991. * Supported in part by grant HD-24649 from the National Institutes of Health, Bethesda, Maryland. t Presented at the 38th Annual Meeting of the Society for Gynecologic Investigation, San Antonio, Texas, March 20 to 23, 1991. *D epartment of Obstetrics and Gynecology, University of Medicine and Dentistry-New Jersey Medical School. § Reprint requests: Jose M. Colon, M.D., Department of Obstetrics and Gynecology, University of Medicine and DentistryNew Jersey Medical School, MSB E-506, 185 South Orange Avenue, Newark, New Jersey 07103. II Present address: 88 University Place, New York, New York. If Electric engineer, Department of Laser Science Research, AT&T Bell Laboratories. # Department of Preventive Medicine, University of Medicine and Dentistry of New Jersey-New Jersey Medical School. ** Department of Biological Science, Rutgers State University of New Jersey.

PY - 1992

Y1 - 1992

N2 - Individual human sperm can be micromanipulated in three dimensions using a 1.06 μm Nd:YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 μm/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.

AB - Individual human sperm can be micromanipulated in three dimensions using a 1.06 μm Nd:YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 μm/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.

KW - Laser optical trap

KW - human sperm

KW - sperm velocity

UR - https://www.scopus.com/pages/publications/0026548093

U2 - 10.1016/S0015-0282(16)54926-7

DO - 10.1016/S0015-0282(16)54926-7

M3 - Article

C2 - 1740221

AN - SCOPUS:0026548093

SN - 0015-0282

VL - 57

SP - 695

EP - 698

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 3

ER -

Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: Effect on sperm velocity

Abstract

Keywords

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