Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin

Saul Puszkin; Elena Puszkin; Arnold M. Katz; Louis M. Aledort

doi:10.1016/0005-2728(74)90203-5

Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin

Saul Puszkin
, Elena Puszkin
, Arnold M. Katz
, Louis M. Aledort

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

1. 1. Platelet actomyosin superprecipitation performed in 0.09 M to 0.12 M KCl showed a biphasic curve with clearing and turbidity increase after addition of Mg-ATP. 2. 2. Addition of ADP produced inhibition of the duration and extent of the clearing phase (K_m of 4 · 10^-5 M), and activation of the superprecipitation phase (K_m of 1.96 · 10^-5 M). 3. 3. The effect of ADP was observed with no significant increase of ATPase activity and was apparently specific for ADP and not for GDP, UDP, or IDP when superprecipitation was initiated only with Mg-ATP. 4. 4. The effect of ADP was observed when superprecipitation occurred in the presence of EGTA. These data suggest that ADP may form part of a control mechanism of platelet contractile activity.

Original language	English
Pages (from-to)	102-112
Number of pages	11
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	347
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(74)90203-5
State	Published - 23 Apr 1974
Externally published	Yes

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@article{a9e868b832d24c56968f51930d1c1eaa,

title = "Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin",

abstract = "1. 1. Platelet actomyosin superprecipitation performed in 0.09 M to 0.12 M KCl showed a biphasic curve with clearing and turbidity increase after addition of Mg-ATP. 2. 2. Addition of ADP produced inhibition of the duration and extent of the clearing phase (Km of 4 · 10-5 M), and activation of the superprecipitation phase (Km of 1.96 · 10-5 M). 3. 3. The effect of ADP was observed with no significant increase of ATPase activity and was apparently specific for ADP and not for GDP, UDP, or IDP when superprecipitation was initiated only with Mg-ATP. 4. 4. The effect of ADP was observed when superprecipitation occurred in the presence of EGTA. These data suggest that ADP may form part of a control mechanism of platelet contractile activity.",

author = "Saul Puszkin and Elena Puszkin and Katz, \{Arnold M.\} and Aledort, \{Louis M.\}",

note = "Funding Information: We thank Rosalba Maldonado Mufioz, Neil Abis and Daniel Hochberger for their invaluable technical assistance. We also thank Dr S. Kochwa for helpful discussions. This work was supported by USPHS Grants HL 12443, HL 10905, HL 13191, CA 11816, HD 03972 and HL 05488 and by The New York Heart Association.",

year = "1974",

month = apr,

day = "23",

doi = "10.1016/0005-2728(74)90203-5",

language = "English",

volume = "347",

pages = "102--112",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Control of platelet actomyosin activity

T2 - Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin

AU - Puszkin, Saul

AU - Puszkin, Elena

AU - Katz, Arnold M.

AU - Aledort, Louis M.

N1 - Funding Information: We thank Rosalba Maldonado Mufioz, Neil Abis and Daniel Hochberger for their invaluable technical assistance. We also thank Dr S. Kochwa for helpful discussions. This work was supported by USPHS Grants HL 12443, HL 10905, HL 13191, CA 11816, HD 03972 and HL 05488 and by The New York Heart Association.

PY - 1974/4/23

Y1 - 1974/4/23

N2 - 1. 1. Platelet actomyosin superprecipitation performed in 0.09 M to 0.12 M KCl showed a biphasic curve with clearing and turbidity increase after addition of Mg-ATP. 2. 2. Addition of ADP produced inhibition of the duration and extent of the clearing phase (Km of 4 · 10-5 M), and activation of the superprecipitation phase (Km of 1.96 · 10-5 M). 3. 3. The effect of ADP was observed with no significant increase of ATPase activity and was apparently specific for ADP and not for GDP, UDP, or IDP when superprecipitation was initiated only with Mg-ATP. 4. 4. The effect of ADP was observed when superprecipitation occurred in the presence of EGTA. These data suggest that ADP may form part of a control mechanism of platelet contractile activity.

AB - 1. 1. Platelet actomyosin superprecipitation performed in 0.09 M to 0.12 M KCl showed a biphasic curve with clearing and turbidity increase after addition of Mg-ATP. 2. 2. Addition of ADP produced inhibition of the duration and extent of the clearing phase (Km of 4 · 10-5 M), and activation of the superprecipitation phase (Km of 1.96 · 10-5 M). 3. 3. The effect of ADP was observed with no significant increase of ATPase activity and was apparently specific for ADP and not for GDP, UDP, or IDP when superprecipitation was initiated only with Mg-ATP. 4. 4. The effect of ADP was observed when superprecipitation occurred in the presence of EGTA. These data suggest that ADP may form part of a control mechanism of platelet contractile activity.

UR - https://www.scopus.com/pages/publications/0016234276

U2 - 10.1016/0005-2728(74)90203-5

DO - 10.1016/0005-2728(74)90203-5

M3 - Article

C2 - 4279699

AN - SCOPUS:0016234276

SN - 0005-2728

VL - 347

SP - 102

EP - 112

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

IS - 1

ER -

Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin

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