Construction of multi-ribozyme expression system and its characterization of cleavage on the MDR1/MRP1 double target substrate in vitro

Keyphrases

• Ribozyme 100%
• Multidrug Resistance Protein 1 (MDR1) 100%
• Expression System 100%
• Double Target 100%
• Hammerhead Ribozyme 41%
• Mg2+ 41%
• Trans-acting 25%
• Cis-acting 16%
• Target RNA 16%
• RNA Substrates 16%
• DNA Sequencing 8%
• Catalytic Activity 8%
• Plasmid 8%
• Ion Concentration 8%
• Linear Relationship 8%
• Cell-free System 8%
• Recombinant Plasmid 8%
• DNA Recombination 8%
• Self-cleavage 8%
• Cleavage Reactions 8%
• Nuclease Digestion 8%
• Cleavage Activity 8%
• Cleavage Efficiency 8%

Engineering

• Expression System 100%
• Ion Concentration 20%
• Enzymatic Activity 20%
• Free System 20%
• Linear Relationship 20%
• Target Site 20%

Chemistry

• RNA 100%
• Deoxyribonucleic Acid 33%
• DNA Sequencing 33%
• Catalyst Activity 33%