The first and very preliminary linkage map of the jute genome was constructed with eight ISSR (Inter Simple Sequence Repeat) markers and their different combinations using an F2 population developed from a cross between jute cultivars O-9897 and Acc. 1805 which differed in cold tolerance. Linkage analyses at a LOD [logarithm of the odds (to the base 10)] score of 3.0 and a maximum distance 50.0 revealed three linkage groups. The analysis revealed three linkage groups varying in length from 4.8 to 52.9 cM. The intraspecific map spanned a total length of 87.3 cM with an average marker density of 8.73 cM between adjacent markers. The utility of ISSR markers for mapping in jute was explored and the primer with a CTT repeat motif was found to be useful. ISSR primers were found to be 50% polymorphic between the parent cultivars.
- Linkage map