Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

Benjamin Gantenbein-Ritter; Christoph M. Sprecher; Samantha Chan; Svenja Illien-Jünger; Sibylle Grad

doi:10.1007/978-1-61779-108-6_14

Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, Sibylle Grad

Icahn School of Medicine at Mount Sinai

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

19 Scopus citations

Abstract

In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	127-140
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-61779-108-6_14
State	Published - 2011

Publication series

Name	Methods in Molecular Biology
Volume	740
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Calcein AM
Cell viability
Confocal laser scanning microscopy
Ethidium homodimer-1
Hydrogel
PKH26 tracker dye
Tissue

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10.1007/978-1-61779-108-6_14

Cite this

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title = "Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers",

abstract = "In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.",

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author = "Benjamin Gantenbein-Ritter and Sprecher, {Christoph M.} and Samantha Chan and Svenja Illien-J{\"u}nger and Sibylle Grad",

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language = "English",

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T1 - Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

AU - Gantenbein-Ritter, Benjamin

AU - Sprecher, Christoph M.

AU - Chan, Samantha

AU - Illien-Jünger, Svenja

AU - Grad, Sibylle

PY - 2011

Y1 - 2011

N2 - In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.

AB - In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.

KW - Calcein AM

KW - Cell viability

KW - Confocal laser scanning microscopy

KW - Ethidium homodimer-1

KW - Hydrogel

KW - PKH26 tracker dye

KW - Tissue

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M3 - Chapter

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AN - SCOPUS:79960127934

T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

Abstract

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