TY - JOUR
T1 - Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology
AU - Qi, Xiaoli
AU - Muñoz-Castañeda, Rodrigo
AU - Yue, Yuanlei
AU - Xiao, Sheng
AU - Ding, Liya
AU - Narasimhan, Arun
AU - Chen, Xin
AU - Wang, Wei
AU - Li, Yuanyuan
AU - Elowsky, Corey
AU - Palmer, Jason
AU - Drewes, Rhonda
AU - Sun, Jianjun
AU - Wu, Jinglei
AU - Qu, Lei
AU - Wu, Zhuhao
AU - Peng, Hanchuan
AU - Osten, Pavel
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Nature America, Inc. 2025.
PY - 2025/12
Y1 - 2025/12
N2 - Advanced brain-wide mapping is critical for addressing complex questions in neuroscience. However, current imaging methods are limited by throughput, resolution and signal-to-noise ratio, constraining their broader applicability. Here, we present confocal Airy beam integrated with single-photon oblique light-sheet tomography (CAB-OLST): a system that integrates single-photon excitation with a scanned Airy beam light sheet, virtual slit detection and automated mechanical sectioning. CAB-OLST enables high-throughput, high-resolution and high-signal-to-noise ratio volumetric imaging, achieving an optical resolution of 0.77 μm × 0.49 μm × 2.61 μm. This allows for mouse brain-wide cell type distribution mapping at a voxel size of 0.37 μm × 0.37 μm × 1.77 μm in 10 h and single-neuron projectome imaging with a voxel size of 0.26 μm × 0.26 μm × 1.06 μm over 58 h. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.
AB - Advanced brain-wide mapping is critical for addressing complex questions in neuroscience. However, current imaging methods are limited by throughput, resolution and signal-to-noise ratio, constraining their broader applicability. Here, we present confocal Airy beam integrated with single-photon oblique light-sheet tomography (CAB-OLST): a system that integrates single-photon excitation with a scanned Airy beam light sheet, virtual slit detection and automated mechanical sectioning. CAB-OLST enables high-throughput, high-resolution and high-signal-to-noise ratio volumetric imaging, achieving an optical resolution of 0.77 μm × 0.49 μm × 2.61 μm. This allows for mouse brain-wide cell type distribution mapping at a voxel size of 0.37 μm × 0.37 μm × 1.77 μm in 10 h and single-neuron projectome imaging with a voxel size of 0.26 μm × 0.26 μm × 1.06 μm over 58 h. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.
UR - https://www.scopus.com/pages/publications/105021525212
U2 - 10.1038/s41592-025-02888-9
DO - 10.1038/s41592-025-02888-9
M3 - Article
AN - SCOPUS:105021525212
SN - 1548-7091
VL - 22
SP - 2622
EP - 2630
JO - Nature Methods
JF - Nature Methods
IS - 12
ER -