Conductivity detection for molecular mass estimation of per-O-sulfonated glycosaminoglycans separated by high-performance size-exclusion chromatography

Amornrut Chaidedgumjorn, Atsushi Suzuki, Hidenao Toyoda, Toshihiko Toida, Toshio Imanari, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Chemically per-O-sulfonated polysaccharides, including glycosaminoglycans (GAGs) and hyaluronan oligosaccharides were analyzed using high-performance size-exclusion chromatography (HPSEC) with suppressed conductivity detection. The results were compared to those obtained by gel filtration HPLC using UV detection or fluorescence detection after the post-column reaction with 2-cyanoacetamide in strong alkaline solution. Analysis was performed on a TSKgel G3000SWXL HPSEC column in 5 mM boric acid (pH 7.0 adjusted by 10 mM NaOH). The use of conductivity detection, in the absence of any derivatization and under isocratic conditions gave a limit of detection in the picogram range. Preliminary studies suggest that this approach may be particularly useful in examining sulfonated polysaccharides and oligosaccharides having no UV chromophore, such as those prepared from O-sulfonated fucans and galactans isolated from algae.

Original languageEnglish
Article number33471
Pages (from-to)95-102
Number of pages8
JournalJournal of Chromatography A
Volume959
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Glycosaminoglycams
  • Oligosaccharides
  • Polysaccharides

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