Computational Approaches for the Investigation of Intra-tumor Heterogeneity and Clonal Evolution from Bulk Sequencing Data in Precision Oncology Applications

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

While the clonal model of cancer evolution was first proposed over 40 years ago, only recently next-generation sequencing has allowed a more precise and quantitative assessment of tumor clonal and subclonal landscape. Consequently, a plethora of computational approaches and tools have been developed to analyze this data with the goal of inferring the clonal landscape of a tumor and characterize its temporal or spatial evolution. This chapter introduces intra-tumor heterogeneity (ITH) in the context of precision oncology applications and provides an overview of the basic concepts, algorithms, and tools for the dissection, analysis, and visualization of ITH from bulk DNA sequencing.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer
Pages101-118
Number of pages18
DOIs
StatePublished - 2022

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1361
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Cancer
  • Clonal expansion
  • Clonality
  • Clone
  • DNA sequencing
  • ITH
  • NGS
  • Subclone
  • Tumor
  • Tumor evolution
  • Tumor phylogeny

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