TY - JOUR
T1 - Comprehensive analysis of glycosaminoglycans from the edible shellfish
AU - Okamoto, Yusuke
AU - Higashi, Kyohei
AU - Linhardt, Robert J.
AU - Toida, Toshihiko
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/3/15
Y1 - 2018/3/15
N2 - We have previously reported that the keratan sulfate (KS) disaccharide was branched to the C-3 position of glucuronate in chondroitin sulfate (CS)-E derived from the Mactra chinensis. We carried out the comprehensive disaccharide analysis of GAGs from 10 shellfish, Ruditapes philippinarum, Scapharca broughtonii, Mizuhopecten yessoensis, Turbo cornutus, Crassostrea nippona, Corbicula japonica, Mytilus galloprovincialis, Neptunea intersculpta, Pseudocardium sachalinense and Crassostrea gigas, to better understand the glycan structures in marine organisms. The contents of CS, heparan sulfate and hyaluronic acid and their compositions depend on the species of shellfish. Interestingly, a peak corresponding to a pentasaccharide containing KS disaccharide was observed when GAGs from T. cornutus was treated with chondroitinase (Chase) ACII but not Chase ABC. In addition, unidentified peaks were also observed when CS derived from R. philippinarum, S. broughtonii were treated with Chase ACII. These results suggest the presence of additional unidentified structure of CS in these shellfish.
AB - We have previously reported that the keratan sulfate (KS) disaccharide was branched to the C-3 position of glucuronate in chondroitin sulfate (CS)-E derived from the Mactra chinensis. We carried out the comprehensive disaccharide analysis of GAGs from 10 shellfish, Ruditapes philippinarum, Scapharca broughtonii, Mizuhopecten yessoensis, Turbo cornutus, Crassostrea nippona, Corbicula japonica, Mytilus galloprovincialis, Neptunea intersculpta, Pseudocardium sachalinense and Crassostrea gigas, to better understand the glycan structures in marine organisms. The contents of CS, heparan sulfate and hyaluronic acid and their compositions depend on the species of shellfish. Interestingly, a peak corresponding to a pentasaccharide containing KS disaccharide was observed when GAGs from T. cornutus was treated with chondroitinase (Chase) ACII but not Chase ABC. In addition, unidentified peaks were also observed when CS derived from R. philippinarum, S. broughtonii were treated with Chase ACII. These results suggest the presence of additional unidentified structure of CS in these shellfish.
KW - Disaccharide composition
KW - Edible shellfish
KW - Glycosaminoglycans
KW - New structures
UR - http://www.scopus.com/inward/record.url?scp=85039856452&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2017.12.076
DO - 10.1016/j.carbpol.2017.12.076
M3 - Article
C2 - 29352919
AN - SCOPUS:85039856452
SN - 0144-8617
VL - 184
SP - 269
EP - 276
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -