Complex synthetic chemical libraries indexed with molecular tags

Michael H.J. Ohlmeyer; Robert N. Swanson; Lawrence W. Dillard; John C. Reader; Gigi Asouline; Ryuji Kobayashi; Michael Wigler; W. Clark Still

Complex synthetic chemical libraries indexed with molecular tags

Michael H.J. Ohlmeyer, Robert N. Swanson, Lawrence W. Dillard, John C. Reader, Gigi Asouline, Ryuji Kobayashi, Michael Wigler, W. Clark Still

Research output: Contribution to journal › Article › peer-review

534 Scopus citations

Abstract

Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.

Original language	English
Pages (from-to)	10922-10926
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	23
State	Published - 1 Dec 1993
Externally published	Yes

Keywords

Antibody recognition
Combinatorial chemistry
Encoded libraries
Peptides

Cite this

@article{5430198370904f2e854449a1793a0b1a,

title = "Complex synthetic chemical libraries indexed with molecular tags",

abstract = "Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.",

keywords = "Antibody recognition, Combinatorial chemistry, Encoded libraries, Peptides",

author = "Ohlmeyer, {Michael H.J.} and Swanson, {Robert N.} and Dillard, {Lawrence W.} and Reader, {John C.} and Gigi Asouline and Ryuji Kobayashi and Michael Wigler and Still, {W. Clark}",

year = "1993",

month = dec,

day = "1",

language = "English",

volume = "90",

pages = "10922--10926",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "23",

}

TY - JOUR

T1 - Complex synthetic chemical libraries indexed with molecular tags

AU - Ohlmeyer, Michael H.J.

AU - Swanson, Robert N.

AU - Dillard, Lawrence W.

AU - Reader, John C.

AU - Asouline, Gigi

AU - Kobayashi, Ryuji

AU - Wigler, Michael

AU - Still, W. Clark

PY - 1993/12/1

Y1 - 1993/12/1

N2 - Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.

AB - Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.

KW - Antibody recognition

KW - Combinatorial chemistry

KW - Encoded libraries

KW - Peptides

UR - http://www.scopus.com/inward/record.url?scp=0027362628&partnerID=8YFLogxK

M3 - Article

C2 - 7504286

AN - SCOPUS:0027362628

SN - 0027-8424

VL - 90

SP - 10922

EP - 10926

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 23

ER -

Complex synthetic chemical libraries indexed with molecular tags

Abstract

Keywords

Fingerprint

Cite this