TY - JOUR
T1 - Complete vascular healing and sustained suppression of neointimal thickening after local delivery of advanced c-myc antisense at six months follow-up in a rabbit balloon injury model
AU - Kipshidze, Nicholas
AU - Iversen, Patrick
AU - Keane, Eamon
AU - Stein, David
AU - Chawla, Paramjith
AU - Skrinska, Victor
AU - Shankar, Latha Raja
AU - Mehran, Roxana
AU - Chekanov, Valerie
AU - Dangas, George
AU - Komorowski, Richard
AU - Haudenschild, Christian
AU - Khanna, Ashwani
AU - Leon, Martin
AU - Keelan, Michael H.
AU - Moses, Jeffrey
N1 - Funding Information:
The authors owe much gratitude to John Peterson, MD for his review of the manuscript and to Cathy Kennedy for copyediting and manuscript preparation. These studies were supported in part by AVI-BioPharma (Portland, OR) and the Leon Hess Research Foundation, New York, NY.
PY - 2002/1
Y1 - 2002/1
N2 - Backgroud: Neointimal hyperplasia following percutaneous transluminal coronary angioplasty (PTCA) is one of the major components of the process of restenosis. We evaluated the long-term impact of local delivery of c-myc neutrally charged antisense oligonucleotides (Resten-NG) upon neointimal formation following PTCA in a rabbit model. Methods: PTCA was performed in the iliac arteries of 10 New Zealand white rabbits at 8 atm for 30 s, three times. An infusion of 500 μg Resten-NG (n=6) or saline (n=4) was delivered to the site at 2 atm via the outer balloon pores of the transport™ catheter over 2 min. The diet was supplemented with 0.25% cholesterol for 10 days before and 6 months following PTCA. Results: After 6 months, animals were sacrificed and vessels were fixed in formalin, processed and stained with hematoxylin, eosin, and movat. Histological analysis revealed complete vascular healing in both groups of animals. Planimetry showed that intimal areas were 1.71±0.25 and 0.65±0.36 mm2 in the control and antisense delivery groups, respectively (P<.05). Conclusion: We conclude that local delivery of Resten-NG significantly inhibited neointimal thickening following PTCA in a rabbit for up to 6 months.
AB - Backgroud: Neointimal hyperplasia following percutaneous transluminal coronary angioplasty (PTCA) is one of the major components of the process of restenosis. We evaluated the long-term impact of local delivery of c-myc neutrally charged antisense oligonucleotides (Resten-NG) upon neointimal formation following PTCA in a rabbit model. Methods: PTCA was performed in the iliac arteries of 10 New Zealand white rabbits at 8 atm for 30 s, three times. An infusion of 500 μg Resten-NG (n=6) or saline (n=4) was delivered to the site at 2 atm via the outer balloon pores of the transport™ catheter over 2 min. The diet was supplemented with 0.25% cholesterol for 10 days before and 6 months following PTCA. Results: After 6 months, animals were sacrificed and vessels were fixed in formalin, processed and stained with hematoxylin, eosin, and movat. Histological analysis revealed complete vascular healing in both groups of animals. Planimetry showed that intimal areas were 1.71±0.25 and 0.65±0.36 mm2 in the control and antisense delivery groups, respectively (P<.05). Conclusion: We conclude that local delivery of Resten-NG significantly inhibited neointimal thickening following PTCA in a rabbit for up to 6 months.
UR - http://www.scopus.com/inward/record.url?scp=18744388262&partnerID=8YFLogxK
U2 - 10.1016/S1522-1865(02)00149-X
DO - 10.1016/S1522-1865(02)00149-X
M3 - Article
C2 - 12479913
AN - SCOPUS:18744388262
SN - 1522-1865
VL - 3
SP - 26
EP - 30
JO - Cardiovascular Radiation Medicine
JF - Cardiovascular Radiation Medicine
IS - 1
ER -