Complement receptor in synchronized cultures of human hematopoietic cell lines

Human lymphoid cell lines derived from peripheral blood of patients with a variety of immunologic disorders as well as from normal individuals have been used for the analysis of function and properties of lymphoid cells. These lymphoid cells are lymphoblastoid in appearance, produce immunoglobulins, complement components, interferon, mediators of cellular immunity, HLA antigens, and enzymes. Since these rapidly dividing culture cells can be easily synchronized, various investigators have employed them for the study of the behavior of surface receptors and other key biologic substances in relation to the various metabolic stages of the cell cycle. In the present study the surface complement receptor, thought to be representative of B cells, of the cultured lymphoid cell was investigated in relation to the cell cycle with synchronized human long term lymphoid cell lines.