Comparison of results from different laboratories in measuring 8-oxo-2′-deoxyguanosine in synthetic oligonucleotides

Bente Riis, Andrew Collins, Catherine Gedik, Sharon Wood, Ann White, Jacques Dubois, Pierre Duez, Jean François Rees, Rozenn Legall, Liliane Degand, Steffen Loft, Anna Hansen, Henrik Enghusen Poulsen, Allan Weimann, Bente Riis Jensen, Jean Cadet, Thierry Douki, Jean Luc Ravanat, Henry Faure, Michele TripierIsabelle Morel, Odile Sergent, Pierre Cillard, Bénédicte Morin, Bernd Epe, Nicole Phoa, Andrea Hartwig, Anke Pelzer, Piero Dolara, Chiara Casalini, Francesco Guglielmi, Cristina Luceri, Hiroshi Kasai, Rie Kido, Ryszard Olinski, Karol Bialkowski, Zdena Durackova, Lucia Hlincikova, Peter Korytar, Mária Dušinská, Csilla Mislanova, José Viña, Ana Lloret, Lennart Möller, Tim Hofer, Eric Gremaud, Laurent Fay, Richard Stadler, Julie Eakins, François Pognan, John O'Brien, Ruan Elliott, Siân Astley, Angela Bailley, Karl Herbert, Dina Chauhan, Frank Kelly, Chrisi Dunster, Joseph Lunec, Ian Podmore, Parul Patel, Samantha Johnson, Mark Evans, Andrea White, Rex Tyrrell, Matthew Gordon, Chris Wild, Laura Hardie, Elizabeth Smith

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up in 1997 to resolve methodological problems and to reach agreement on the basal level of 8-oxo-2′-deoxyguanosine (8-oxodG) in biological samples. In the present ESCODD trial 6 samples of 8-oxodG-containing oligonucleotides with different ratios of 8-oxodG/2′-deoxyadenosine (dAdo) were sent to 25 laboratories throughout Europe. The methods used were HPLC with electrochemical detection (amperometric or coulometric), GC-MS or LC-MS-MS. The LC-MS-MS and the coulometric HPLC analyses gave 8-oxodG concentrations within 53 and 73% of expected values, respectively, whereas the amperometric HPLC and GC-MS consistently overestimated the 8-oxodG concentration by several fold. As the oligonucleotides contained no 2′-deoxyguanosine (dGuo), this was not due to artificial oxidation. On the contrary, in most cases the concentrations of dAdo and thymidine (dThd), used as estimates for non-oxidised DNA bases were underestimated, though a few laboratories overestimated the lowest concentration samples containing 8 and 20 μM, respectively. In one-third of the reported results, the ratio of 8-oxodG/10 5 dAdo was within 25% of the calculated value in the oligonucleotide samples and in half of the results the coefficient of variation in duplicate samples was less than 10%. The coefficients of variation were higher for the dAdo concentrations than for 8-oxodG. Our findings strongly indicate that careful quality control must be applied to the analytical procedures for 8-oxodG and very importantly also to the procedures for non-modified 2′-deoxyribonucleosides. We recommend the use of synthetic oligonucleotides for this purpose.

Original languageEnglish
Pages (from-to)649-659
Number of pages11
JournalFree Radical Research
Issue number6
StatePublished - Jun 2002


  • 8-oxo-2′-deoxyguanosine
  • GC-MS
  • HPLC
  • LC-MS-MS
  • Oxidative DNA damage
  • Standardisation


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