Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas

Jean Pierre Raufman; John Eng; B. H. Du; Eugene Straus; Rosalyn S. Yalow

doi:10.1016/0167-0115(86)90208-9

Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas

Jean Pierre Raufman
, John Eng
, B. H. Du
, Eugene Straus
, Rosalyn S. Yalow

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of ¹²⁵I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.

Original language	English
Pages (from-to)	93-97
Number of pages	5
Journal	Regulatory Peptides
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/0167-0115(86)90208-9
State	Published - Mar 1986
Externally published	Yes

Keywords

acini
guinea pig
mammalian VIP
pancreas

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10.1016/0167-0115(86)90208-9

Cite this

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title = "Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas",

abstract = "Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of 125I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.",

keywords = "acini, guinea pig, mammalian VIP, pancreas",

author = "Raufman, \{Jean Pierre\} and John Eng and Du, \{B. H.\} and Eugene Straus and Yalow, \{Rosalyn S.\}",

note = "Funding Information: Dr. Raufman is a recipient of the AGA/Merrell-Dow Research Scholar Award. Dr. Du is a Fellow of the Solomon A. Berson Fund for Medical Research, Inc. This work was supported in part by the Medical Research Program of the Veterans Administration.",

year = "1986",

month = mar,

doi = "10.1016/0167-0115(86)90208-9",

language = "English",

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TY - JOUR

T1 - Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas

AU - Raufman, Jean Pierre

AU - Eng, John

AU - Du, B. H.

AU - Straus, Eugene

AU - Yalow, Rosalyn S.

N1 - Funding Information: Dr. Raufman is a recipient of the AGA/Merrell-Dow Research Scholar Award. Dr. Du is a Fellow of the Solomon A. Berson Fund for Medical Research, Inc. This work was supported in part by the Medical Research Program of the Veterans Administration.

PY - 1986/3

Y1 - 1986/3

N2 - Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of 125I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.

AB - Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of 125I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.

KW - acini

KW - guinea pig

KW - mammalian VIP

KW - pancreas

UR - https://www.scopus.com/pages/publications/0022640551

U2 - 10.1016/0167-0115(86)90208-9

DO - 10.1016/0167-0115(86)90208-9

M3 - Article

C2 - 3012658

AN - SCOPUS:0022640551

SN - 0167-0115

VL - 14

SP - 93

EP - 97

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 1

ER -

Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas

Abstract

Keywords

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