TY - JOUR
T1 - Comparison of mammalian VIP bioactivities in dispersed acini from guinea pig pancreas
AU - Raufman, Jean Pierre
AU - Eng, John
AU - Du, B. H.
AU - Straus, Eugene
AU - Yalow, Rosalyn S.
N1 - Funding Information:
Dr. Raufman is a recipient of the AGA/Merrell-Dow Research Scholar Award. Dr. Du is a Fellow of the Solomon A. Berson Fund for Medical Research, Inc. This work was supported in part by the Medical Research Program of the Veterans Administration.
PY - 1986/3
Y1 - 1986/3
N2 - Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of 125I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.
AB - Mammalian VIP is identical in pig, cow, human, rat, dog and goat but differs in the guinea pig (GP) in positions 5, 9, 19, and 26. We now demonstrate that GP, goat, rat and synthetic mammalian VIP are indistinguishable in their inhibition of binding of 125I-labelled synthetic VIP to dispersed acini from GP pancreas and that GP, pig, dog, goat and synthetic VIP are also similar in their efficacy and potency in stimulating amylase release from these acini. Thus in spite of the differences in amino acid sequence, GP VIP appears to have full biologic potency in its action on dispersed acini from GP pancreas.
KW - acini
KW - guinea pig
KW - mammalian VIP
KW - pancreas
UR - http://www.scopus.com/inward/record.url?scp=0022640551&partnerID=8YFLogxK
U2 - 10.1016/0167-0115(86)90208-9
DO - 10.1016/0167-0115(86)90208-9
M3 - Article
C2 - 3012658
AN - SCOPUS:0022640551
SN - 0167-0115
VL - 14
SP - 93
EP - 97
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 1
ER -