Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively

Ji Ping Zheng; Ling Chun Wang; Peng Wang; Gang Luo; Shu Qin Li; Zhao Shan Zhang; Cui Fen Huang

Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively

Ji Ping Zheng
, Ling Chun Wang
, Peng Wang
, Gang Luo
, Shu Qin Li
, Zhao Shan Zhang
, Cui Fen Huang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Objective: To select and develop an effective and safe attenuated vaccine against ETEC. Methods: A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co-expressing colonization factor antigen I (CFA/ I) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/ I and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses. Results: Animals immunized with above strains developed specific serum antibodies and secretory immunoglobulin A(sIgA) aganist CFA/ I and CS6 of ETEC. The antibody titers of the group only immunized with attenuated Shigella flexneri serum FWL01(pZCF16) are similar or higher than the group immunized with the mixture of two strains FWL01(pZHY21) and FWL01(pZLG6). Conclusions: These results show the feasibility of a multivalent vaccine expressing different fimbriae antigens in a live carrier.

Original language	English
Pages (from-to)	209-213
Number of pages	5
Journal	Chinese Journal of Microbiology and Immunology
Volume	24
Issue number	3
State	Published - Mar 2004

Keywords

CFA/ I
CS6
Enterotoxigenic E. coli
Immunization
Shigella flexneri

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Zheng, J. P., Wang, L. C., Wang, P., Luo, G., Li, S. Q., Zhang, Z. S., & Huang, C. F. (2004). Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively. Chinese Journal of Microbiology and Immunology, 24(3), 209-213.

Zheng, Ji Ping ; Wang, Ling Chun ; Wang, Peng et al. / Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively. In: Chinese Journal of Microbiology and Immunology. 2004 ; Vol. 24, No. 3. pp. 209-213.

@article{f3a13a0199384158ac5eaa6d562e2bc1,

title = "Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively",

abstract = "Objective: To select and develop an effective and safe attenuated vaccine against ETEC. Methods: A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co-expressing colonization factor antigen I (CFA/ I) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/ I and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses. Results: Animals immunized with above strains developed specific serum antibodies and secretory immunoglobulin A(sIgA) aganist CFA/ I and CS6 of ETEC. The antibody titers of the group only immunized with attenuated Shigella flexneri serum FWL01(pZCF16) are similar or higher than the group immunized with the mixture of two strains FWL01(pZHY21) and FWL01(pZLG6). Conclusions: These results show the feasibility of a multivalent vaccine expressing different fimbriae antigens in a live carrier.",

keywords = "CFA/ I, CS6, Enterotoxigenic E. coli, Immunization, Shigella flexneri",

author = "Zheng, \{Ji Ping\} and Wang, \{Ling Chun\} and Peng Wang and Gang Luo and Li, \{Shu Qin\} and Zhang, \{Zhao Shan\} and Huang, \{Cui Fen\}",

year = "2004",

month = mar,

language = "English",

volume = "24",

pages = "209--213",

journal = "Chinese Journal of Microbiology and Immunology",

issn = "0254-5101",

publisher = "Chinese Medical Journals Publishing House Co.Ltd",

number = "3",

}

Zheng, JP, Wang, LC, Wang, P, Luo, G, Li, SQ, Zhang, ZS & Huang, CF 2004, 'Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively', Chinese Journal of Microbiology and Immunology, vol. 24, no. 3, pp. 209-213.

Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively. / Zheng, Ji Ping; Wang, Ling Chun; Wang, Peng et al.
In: Chinese Journal of Microbiology and Immunology, Vol. 24, No. 3, 03.2004, p. 209-213.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively

AU - Zheng, Ji Ping

AU - Wang, Ling Chun

AU - Wang, Peng

AU - Luo, Gang

AU - Li, Shu Qin

AU - Zhang, Zhao Shan

AU - Huang, Cui Fen

PY - 2004/3

Y1 - 2004/3

N2 - Objective: To select and develop an effective and safe attenuated vaccine against ETEC. Methods: A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co-expressing colonization factor antigen I (CFA/ I) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/ I and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses. Results: Animals immunized with above strains developed specific serum antibodies and secretory immunoglobulin A(sIgA) aganist CFA/ I and CS6 of ETEC. The antibody titers of the group only immunized with attenuated Shigella flexneri serum FWL01(pZCF16) are similar or higher than the group immunized with the mixture of two strains FWL01(pZHY21) and FWL01(pZLG6). Conclusions: These results show the feasibility of a multivalent vaccine expressing different fimbriae antigens in a live carrier.

AB - Objective: To select and develop an effective and safe attenuated vaccine against ETEC. Methods: A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co-expressing colonization factor antigen I (CFA/ I) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/ I and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses. Results: Animals immunized with above strains developed specific serum antibodies and secretory immunoglobulin A(sIgA) aganist CFA/ I and CS6 of ETEC. The antibody titers of the group only immunized with attenuated Shigella flexneri serum FWL01(pZCF16) are similar or higher than the group immunized with the mixture of two strains FWL01(pZHY21) and FWL01(pZLG6). Conclusions: These results show the feasibility of a multivalent vaccine expressing different fimbriae antigens in a live carrier.

KW - CFA/ I

KW - CS6

KW - Enterotoxigenic E. coli

KW - Immunization

KW - Shigella flexneri

UR - https://www.scopus.com/pages/publications/3042720199

M3 - Article

AN - SCOPUS:3042720199

SN - 0254-5101

VL - 24

SP - 209

EP - 213

JO - Chinese Journal of Microbiology and Immunology

JF - Chinese Journal of Microbiology and Immunology

IS - 3

ER -

Zheng JP, Wang LC, Wang P, Luo G, Li SQ, Zhang ZS et al. Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/ I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/ I CS6 respectively. Chinese Journal of Microbiology and Immunology. 2004 Mar;24(3):209-213.