Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay

K. Bromberg, G. Tannis, B. Daidone, L. Clarke, M. Sierra

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in children. Rapid identification of RSV infections would allow for specific chemotherapy. We evaluated a means of rapid diagnosis, the Abbott enzyme immunoassay (EIA), by using 314 stored nasopharyngeal aspirates. RSV antigens were identified in 62 of 66 RSV culture-positive specimens. An additional 37 specimens from which RSV was not isolated were positive in the EIA. Of these, 29 were confirmed as truly positive by a blocking assay, for a total of 95 (66 + 29) positive specimens. The sensitivity of the EIA for total positive samples was 96% (91/95) versus 69% (66/95) for cell culture. The specificity of the EIA was 96% (211/219). In these stored specimens, Abbott EIA was superior to cell culture for the detection of RSV.

Original languageEnglish
Pages (from-to)434-436
Number of pages3
JournalJournal of Clinical Microbiology
Volume25
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

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