TY - JOUR
T1 - Comparison of DNA adduct levels in nasal mucosa, lymphocytes and bronchial mucosa of cigarette smokers and interaction with metabolic gene polymorphisms
AU - Peluso, Marco
AU - Neri, Monica
AU - Margarino, Giovanni
AU - Mereu, Carlo
AU - Munnia, Armelle
AU - Ceppi, Marcello
AU - Buratti, Marina
AU - Felletti, Raffaella
AU - Stea, Francesca
AU - Quaglia, Roberto
AU - Puntoni, Riccardo
AU - Taioli, Emanuela
AU - Garte, Seymour
AU - Bonassi, Stefano
N1 - Funding Information:
We are indebted to Alessandro Blanco and Simonetta Venturi for the efficiency in coordinating sample collection and for their dedication in the relationships with patients. This work was supported by Associazione Italiana per la Ricerca sul Cancro (AIRC) and the European Union V FP (QLRT-2000-00628 and QLRT-2001-02831).
PY - 2004/12
Y1 - 2004/12
N2 - The recent introduction of biomarkers in population studies of lung cancer has improved the traditional epidemiological approach, especially in the detection of high risk groups. Many inhalable carcinogens form DNA adducts, an initial event in lung carcinogenesis, and therefore the identification of easily accessible sources of DNA for population studies is considered a leading priority in the field. In this study we compared the frequency of DNA adducts in samples from nasal brushing, bronchial biopsy and peripheral blood lymphocytes (PBL) in a group of 55 subjects, both smokers and non-smokers, undergoing bronchoscopy for diagnostic purposes. Polymorphisms in the CYP1A1, GSTM1 and GSTT1 genes were also evaluated. The level of DNA adducts measured by 32P-labelling assay in nasal mucosa (108 relative adduct level, mean ± SD 1.10 ± 0.66) was higher than in bronchial mucosa (0.82 ± 0.36) and in PBL (0.54 ± 0.39, P < 0.01). DNA adducts measured in nasal mucosa and in PBL were correlated with those in bronchial mucosa (P < 0.01 and P < 0.05, respectively). DNA adducts in smokers were significantly increased in both nasal mucosa and PBL, with a significant dose-response linear trend (P < 0.05). No significant effect on DNA adduction of the genetic polymorphisms investigated was found. Nasal mucosa brushing proved to be a suitable procedure for the 32P-labelling assay and its use in population studies should be further explored.
AB - The recent introduction of biomarkers in population studies of lung cancer has improved the traditional epidemiological approach, especially in the detection of high risk groups. Many inhalable carcinogens form DNA adducts, an initial event in lung carcinogenesis, and therefore the identification of easily accessible sources of DNA for population studies is considered a leading priority in the field. In this study we compared the frequency of DNA adducts in samples from nasal brushing, bronchial biopsy and peripheral blood lymphocytes (PBL) in a group of 55 subjects, both smokers and non-smokers, undergoing bronchoscopy for diagnostic purposes. Polymorphisms in the CYP1A1, GSTM1 and GSTT1 genes were also evaluated. The level of DNA adducts measured by 32P-labelling assay in nasal mucosa (108 relative adduct level, mean ± SD 1.10 ± 0.66) was higher than in bronchial mucosa (0.82 ± 0.36) and in PBL (0.54 ± 0.39, P < 0.01). DNA adducts measured in nasal mucosa and in PBL were correlated with those in bronchial mucosa (P < 0.01 and P < 0.05, respectively). DNA adducts in smokers were significantly increased in both nasal mucosa and PBL, with a significant dose-response linear trend (P < 0.05). No significant effect on DNA adduction of the genetic polymorphisms investigated was found. Nasal mucosa brushing proved to be a suitable procedure for the 32P-labelling assay and its use in population studies should be further explored.
UR - http://www.scopus.com/inward/record.url?scp=10344252765&partnerID=8YFLogxK
U2 - 10.1093/carcin/bgh259
DO - 10.1093/carcin/bgh259
M3 - Article
C2 - 15319297
AN - SCOPUS:10344252765
SN - 0143-3334
VL - 25
SP - 2459
EP - 2465
JO - Carcinogenesis
JF - Carcinogenesis
IS - 12
ER -