Comparison of adjuvant formulations for cytotoxic T cell induction using synthetic peptides

Catarina E. Hioe, Howard Qiu, Pei De Chend, Zuning Bian, Ming Lie Li, Joseph Li, Manmohan Singh, Peter Kuebler, Paul McGee, Derek O'Hagan, Tim Zamb, Wayne Koff, Catherine Allsopp, Chang Yi Wang, Douglas F. Nixon

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The have investigated the capacity of synthetic peptides delivered in different adjuvant formulations to induce T cytotoxic lymphocyte (CTL) responses to a class I H-2K(d)-restricted Plasmodium berghei circumsporozoite epitope, CS 252-260. Using three immunogen formulations: soybean emulsion; Montanide ISA720, and lipopeptide (P3-CS), we first evaluated the effects of immunization routes on CTL induction. No CTL response was induced in mice immunized s.c. or i.p. with CS peptide formulated in soybean emulsion. In contrast, immunization with lipopeptide P3-CS either s.c. or i.p. effectively primed for CTL. Interestingly, CS peptide emulsified in Montanide ISA720 induced a CTL response only when delivered s.c. and not i.p., indicating the critical influence of immunization routes on CTL induction. We then compared the effectiveness of eight adjuvant formulations to induce CTL response following a single s.c. immunization. Notably, lipopeptide P3-CS and CS peptide admired with P3 or POE lipid molecules stimulated a vigorous CTL response. However, only mice immunized with P3-CS and CS peptide admixed with P3 molecule generated long-lived CTL which persisted in vivo for 5 months. Thus, based on a simultaneous comparison of the different adjuvant formulations, we demonstrated that the conjugated and unconjugated P3 lipopeptides were the most effective immunogens for eliciting primary and memory CTL in mice.

Original languageEnglish
Pages (from-to)412-418
Number of pages7
JournalVaccine
Volume14
Issue number5
DOIs
StatePublished - Apr 1996
Externally publishedYes

Keywords

  • Adjuvant
  • Circumsporozoite
  • Cytotoxic T lymphocyte
  • Plasmodium berghei

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