TY - JOUR
T1 - Comparison of adaptive optics scanning light ophthalmoscopic fluorescein angiography and offset pinhole imaging
AU - Chui, Toco Y.P.
AU - Dubow, Michael
AU - Pinhas, Alexander
AU - Shah, Nishit
AU - Gan, Alexander
AU - Weitz, Rishard
AU - Sulai, Yusufu N.
AU - Dubra, Alfredo
AU - Rosen, Richard B.
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Recent advances to the adaptive optics scanning light ophthalmoscope (AOSLO) have enabled finer in vivo assessment of the human retinal microvasculature. AOSLO confocal reflectance imaging has been coupled with oral fluorescein angiography (FA), enabling simultaneous acquisition of structural and perfusion images. AOSLO offset pinhole (OP) imaging combined with motion contrast post-processing techniques, are able to create a similar set of structural and perfusion images without the use of exogenous contrast agent. In this study, we evaluate the similarities and differences of the structural and perfusion images obtained by either method, in healthy control subjects and in patients with retinal vasculopathy including hypertensive retinopathy, diabetic retinopathy, and retinal vein occlusion. Our results show that AOSLO OP motion contrast provides perfusion maps comparable to those obtained with AOSLO FA, while AOSLO OP reflectance images provide additional information such as vessel wall fine structure not as readily visible in AOSLO confocal reflectance images. AOSLO OP offers a non-invasive alternative to AOSLO FA without the need for any exogenous contrast agent.
AB - Recent advances to the adaptive optics scanning light ophthalmoscope (AOSLO) have enabled finer in vivo assessment of the human retinal microvasculature. AOSLO confocal reflectance imaging has been coupled with oral fluorescein angiography (FA), enabling simultaneous acquisition of structural and perfusion images. AOSLO offset pinhole (OP) imaging combined with motion contrast post-processing techniques, are able to create a similar set of structural and perfusion images without the use of exogenous contrast agent. In this study, we evaluate the similarities and differences of the structural and perfusion images obtained by either method, in healthy control subjects and in patients with retinal vasculopathy including hypertensive retinopathy, diabetic retinopathy, and retinal vein occlusion. Our results show that AOSLO OP motion contrast provides perfusion maps comparable to those obtained with AOSLO FA, while AOSLO OP reflectance images provide additional information such as vessel wall fine structure not as readily visible in AOSLO confocal reflectance images. AOSLO OP offers a non-invasive alternative to AOSLO FA without the need for any exogenous contrast agent.
UR - http://www.scopus.com/inward/record.url?scp=84896984752&partnerID=8YFLogxK
U2 - 10.1364/BOE.5.001173
DO - 10.1364/BOE.5.001173
M3 - Article
AN - SCOPUS:84896984752
SN - 2156-7085
VL - 5
SP - 1173
EP - 1189
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 4
ER -