TY - JOUR
T1 - Comparative functional genomics identifies unique molecular features of EPSCs
AU - Malik, Vikas
AU - Zang, Ruge
AU - Fuentes-Iglesias, Alejandro
AU - Huang, Xin
AU - Li, Dan
AU - Fidalgo, Miguel
AU - Zhou, Hongwei
AU - Wang, Jianlong
N1 - Funding Information:
We thank Drs. Amander T Clark, Richard A Young, Austin Smith, and Hitoshi Niwa for providing Tfap2a/c dKO, Oct4-degron ESCs, ZHBTC4 ESCs, and 2TS22C ESCs, respectively. This work in the Wang laboratory is funded by grants from the National Institutes of Health (R01HD095938 and R01HD097268) and by contracts from New York State Stem Cell Science (NYSTEM#C35583GG). A Fuentes-Iglesias is the recipient of a fellowship from the MINECO of Spain (BES-2017-082007).
Publisher Copyright:
© 2022 Rockefeller University Press. All rights reserved.
PY - 2022/11
Y1 - 2022/11
N2 - Extended pluripotent or expanded potential stem cells (EPSCs) possess superior developmental potential to embryonic stem cells (ESCs). However, the molecular underpinning of EPSC maintenance in vitro is not well defined. We comparatively studied transcriptome, chromatin accessibility, active histone modification marks, and relative proteomes of ESCs and the two well-established EPSC lines to probe the molecular foundation underlying EPSC developmental potential. Despite some overlapping transcriptomic and chromatin accessibility features, we defined sets of molecular signatures that distinguish EPSCs from ESCs in transcriptional and translational regulation as well as metabolic control. Interestingly, EPSCs show similar reliance on pluripotency factors Oct4, Sox2, and Nanog for self-renewal as ESCs. Our study provides a rich resource for dissecting the regulatory network that governs the developmental potency of EPSCs and exploring alternative strategies to capture totipotent stem cells in culture.
AB - Extended pluripotent or expanded potential stem cells (EPSCs) possess superior developmental potential to embryonic stem cells (ESCs). However, the molecular underpinning of EPSC maintenance in vitro is not well defined. We comparatively studied transcriptome, chromatin accessibility, active histone modification marks, and relative proteomes of ESCs and the two well-established EPSC lines to probe the molecular foundation underlying EPSC developmental potential. Despite some overlapping transcriptomic and chromatin accessibility features, we defined sets of molecular signatures that distinguish EPSCs from ESCs in transcriptional and translational regulation as well as metabolic control. Interestingly, EPSCs show similar reliance on pluripotency factors Oct4, Sox2, and Nanog for self-renewal as ESCs. Our study provides a rich resource for dissecting the regulatory network that governs the developmental potency of EPSCs and exploring alternative strategies to capture totipotent stem cells in culture.
UR - http://www.scopus.com/inward/record.url?scp=85136341117&partnerID=8YFLogxK
U2 - 10.26508/lsa.202201608
DO - 10.26508/lsa.202201608
M3 - Article
C2 - 35961778
AN - SCOPUS:85136341117
SN - 2575-1077
VL - 5
JO - Life Science Alliance
JF - Life Science Alliance
IS - 11
M1 - e202201608
ER -