Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples

Armando Gómez-Ojeda; Sarahi Jaramillo-Ortíz; Katarzyna Wrobel; Kazimierz Wrobel; Gloria Barbosa-Sabanero; Claudia Luevano-Contreras; Maria Pia de la Maza; Jaime Uribarri; Ma Dolores del Castillo; Ma Eugenia Garay-Sevilla

doi:10.1016/j.foodchem.2017.09.098

Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of N^ε-carboxymethyl lysine in food samples

Armando Gómez-Ojeda, Sarahi Jaramillo-Ortíz, Katarzyna Wrobel, Kazimierz Wrobel, Gloria Barbosa-Sabanero, Claudia Luevano-Contreras, Maria Pia de la Maza, Jaime Uribarri, Ma Dolores del Castillo, Ma Eugenia Garay-Sevilla

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

N^ε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.

Original language	English
Pages (from-to)	11-18
Number of pages	8
Journal	Food Chemistry
Volume	243
DOIs	https://doi.org/10.1016/j.foodchem.2017.09.098
State	Published - 15 Mar 2018

Keywords

ELISA
Food
Liquid chromatography – mass spectrometry
N-carboxymethyl lysine

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10.1016/j.foodchem.2017.09.098

Cite this

Gómez-Ojeda, A., Jaramillo-Ortíz, S., Wrobel, K., Wrobel, K., Barbosa-Sabanero, G., Luevano-Contreras, C., de la Maza, M. P., Uribarri, J., del Castillo, M. D., & Garay-Sevilla, M. E. (2018). Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of N^ε-carboxymethyl lysine in food samples. Food Chemistry, 243, 11-18. https://doi.org/10.1016/j.foodchem.2017.09.098

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title = "Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples",

abstract = "Nε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source{\textregistered} kit results were not correlated with those provided by Lamider{\textregistered}. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.",

keywords = "ELISA, Food, Liquid chromatography – mass spectrometry, N-carboxymethyl lysine",

author = "Armando G{\'o}mez-Ojeda and Sarahi Jaramillo-Ort{\'i}z and Katarzyna Wrobel and Kazimierz Wrobel and Gloria Barbosa-Sabanero and Claudia Luevano-Contreras and {de la Maza}, {Maria Pia} and Jaime Uribarri and {del Castillo}, {Ma Dolores} and Garay-Sevilla, {Ma Eugenia}",

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doi = "10.1016/j.foodchem.2017.09.098",

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Gómez-Ojeda, A, Jaramillo-Ortíz, S, Wrobel, K, Wrobel, K, Barbosa-Sabanero, G, Luevano-Contreras, C, de la Maza, MP, Uribarri, J, del Castillo, MD & Garay-Sevilla, ME 2018, 'Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of N^ε-carboxymethyl lysine in food samples', Food Chemistry, vol. 243, pp. 11-18. https://doi.org/10.1016/j.foodchem.2017.09.098

TY - JOUR

T1 - Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples

AU - Gómez-Ojeda, Armando

AU - Jaramillo-Ortíz, Sarahi

AU - Wrobel, Katarzyna

AU - Wrobel, Kazimierz

AU - Barbosa-Sabanero, Gloria

AU - Luevano-Contreras, Claudia

AU - de la Maza, Maria Pia

AU - Uribarri, Jaime

AU - del Castillo, Ma Dolores

AU - Garay-Sevilla, Ma Eugenia

PY - 2018/3/15

Y1 - 2018/3/15

N2 - Nε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.

AB - Nε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.

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