Comparative analysis of HIV-1 and murine leukemia virus three-dimensional nuclear distributions

Valentina Quercioli, Cristina di Primio, Antonio Casini, Lubbertus C.F. Mulder, Lenard S. Vranckx, Doortje Borrenberghs, Rik Gijsbers, Zeger Debyser, Anna Cereseto

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Recent advances in fluorescence microscopy allow three-dimensional analysis of HIV-1 preintegration complexes in the nuclei of infected cells. To extend this investigation to gammaretroviruses, we engineered a fluorescent Moloney murine leukemia virus (MLV) system consisting of MLV-integrase fused to enhanced green fluorescent protein (MLV-IN-EGFP). A comparative analysis of lentiviral (HIV-1) and gammaretroviral (MLV) fluorescent complexes in the nuclei of infected cells revealed their different spatial distributions. This research tool has the potential to achieve new insight into the nuclear biology of these retroviruses.

Original languageEnglish
Pages (from-to)5205-5209
Number of pages5
JournalJournal of Virology
Volume90
Issue number10
DOIs
StatePublished - 1 May 2016

Fingerprint

Dive into the research topics of 'Comparative analysis of HIV-1 and murine leukemia virus three-dimensional nuclear distributions'. Together they form a unique fingerprint.

Cite this