Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus

Andrew Collins, Catherine Gedik, Nicholas Vaughan, Sharon Wood, Ann White, Jacques Dubois, Pierre Duez, Gilles Dehon, Jean François Rees, Steffen Loft, Peter Møller, Henrik Poulsen, Bente Riis, Allan Weimann, Jean Cadet, Thierry Douki, Jean Luc Ravanat, Sylvie Sauvaigo, Henry Faure, Isabelle MorelBénédicte Morin, Bernd Epe, Nicole Phoa, Andrea Hartwig, Anke Pelzer, Piero Dolara, Chiara Casalini, Lisa Giovannelli, Maura Lodovici, Ryszard Olinski, Karol Bialkowski, Marek Foksinski, Daniel Gackowski, Zdena Duračková, Lucia Hlinčiková, Peter Korytar, Monika Sivonová, Mária Dušinká, Csilla Mislanová, José Viña, Ana Lloret, Lennart Möller, Tim Hofer, Jonas Nygren, Eric Gremaud, Karl Herbert, Dina Chauhan, Frank Kelly, Crisi Dunster, Joseph Lunec, Marcus Cooke, Mark Evans, Parul Patel, Ian Podmore, Andrea White, Chris Wild, Jo Olliver, Elizabeth Smith

Research output: Contribution to journalReview articlepeer-review

223 Scopus citations


The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 106 guanines, with a median of 10.47 per 106 guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 106 guanines with a median of 5.23 per 106 guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.98 8-oxoGua per 106 guanines, with a median of 0.79 per 106 guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.

Original languageEnglish
Pages (from-to)2129-2133
Number of pages5
Issue number12
StatePublished - 1 Dec 2002
Externally publishedYes


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