Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus

Andrew Collins; Catherine Gedik; Nicholas Vaughan; Sharon Wood; Ann White; Jacques Dubois; Pierre Duez; Gilles Dehon; Jean François Rees; Steffen Loft; Peter Møller; Henrik Poulsen; Bente Riis; Allan Weimann; Jean Cadet; Thierry Douki; Jean Luc Ravanat; Sylvie Sauvaigo; Henry Faure; Isabelle Morel; Bénédicte Morin; Bernd Epe; Nicole Phoa; Andrea Hartwig; Anke Pelzer; Piero Dolara; Chiara Casalini; Lisa Giovannelli; Maura Lodovici; Ryszard Olinski; Karol Bialkowski; Marek Foksinski; Daniel Gackowski; Zdena Duračková; Lucia Hlinčiková; Peter Korytar; Monika Sivonová; Mária Dušinká; Csilla Mislanová; José Viña; Ana Lloret; Lennart Möller; Tim Hofer; Jonas Nygren; Eric Gremaud; Karl Herbert; Dina Chauhan; Frank Kelly; Crisi Dunster; Joseph Lunec; Marcus Cooke; Mark Evans; Parul Patel; Ian Podmore; Andrea White; Chris Wild; Jo Olliver; Elizabeth Smith

doi:10.1093/carcin/23.12.2129

Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus

Andrew Collins, Catherine Gedik, Nicholas Vaughan, Sharon Wood, Ann White, Jacques Dubois, Pierre Duez, Gilles Dehon, Jean François Rees, Steffen Loft, Peter Møller, Henrik Poulsen, Bente Riis, Allan Weimann, Jean Cadet, Thierry Douki, Jean Luc Ravanat, Sylvie Sauvaigo, Henry Faure, Isabelle MorelBénédicte Morin, Bernd Epe, Nicole Phoa, Andrea Hartwig, Anke Pelzer, Piero Dolara, Chiara Casalini, Lisa Giovannelli, Maura Lodovici, Ryszard Olinski, Karol Bialkowski, Marek Foksinski, Daniel Gackowski, Zdena Duračková, Lucia Hlinčiková, Peter Korytar, Monika Sivonová, Mária Dušinká, Csilla Mislanová, José Viña, Ana Lloret, Lennart Möller, Tim Hofer, Jonas Nygren, Eric Gremaud, Karl Herbert, Dina Chauhan, Frank Kelly, Crisi Dunster, Joseph Lunec, Marcus Cooke, Mark Evans, Parul Patel, Ian Podmore, Andrea White, Chris Wild, Jo Olliver, Elizabeth Smith

Research output: Contribution to journal › Review article › peer-review

223 Scopus citations

Abstract

The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 10⁶ guanines, with a median of 10.47 per 10⁶ guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 10⁶ guanines with a median of 5.23 per 10⁶ guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.98 8-oxoGua per 10⁶ guanines, with a median of 0.79 per 10⁶ guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.

Original language	English
Pages (from-to)	2129-2133
Number of pages	5
Journal	Carcinogenesis
Volume	23
Issue number	12
DOIs	https://doi.org/10.1093/carcin/23.12.2129
State	Published - 1 Dec 2002
Externally published	Yes

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10.1093/carcin/23.12.2129

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Collins, A., Gedik, C., Vaughan, N., Wood, S., White, A., Dubois, J., Duez, P., Dehon, G., Rees, J. F., Loft, S., Møller, P., Poulsen, H., Riis, B., Weimann, A., Cadet, J., Douki, T., Ravanat, J. L., Sauvaigo, S., Faure, H., ... Smith, E. (2002). Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus. Carcinogenesis, 23(12), 2129-2133. https://doi.org/10.1093/carcin/23.12.2129

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title = "Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus",

abstract = "The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 106 guanines, with a median of 10.47 per 106 guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 106 guanines with a median of 5.23 per 106 guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.98 8-oxoGua per 106 guanines, with a median of 0.79 per 106 guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.",

author = "Andrew Collins and Catherine Gedik and Nicholas Vaughan and Sharon Wood and Ann White and Jacques Dubois and Pierre Duez and Gilles Dehon and Rees, {Jean Fran{\c c}ois} and Steffen Loft and Peter M{\o}ller and Henrik Poulsen and Bente Riis and Allan Weimann and Jean Cadet and Thierry Douki and Ravanat, {Jean Luc} and Sylvie Sauvaigo and Henry Faure and Isabelle Morel and B{\'e}n{\'e}dicte Morin and Bernd Epe and Nicole Phoa and Andrea Hartwig and Anke Pelzer and Piero Dolara and Chiara Casalini and Lisa Giovannelli and Maura Lodovici and Ryszard Olinski and Karol Bialkowski and Marek Foksinski and Daniel Gackowski and Zdena Dura{\v c}kov{\'a} and Lucia Hlin{\v c}ikov{\'a} and Peter Korytar and Monika Sivonov{\'a} and M{\'a}ria Du{\v s}ink{\'a} and Csilla Mislanov{\'a} and Jos{\'e} Vi{\~n}a and Ana Lloret and Lennart M{\"o}ller and Tim Hofer and Jonas Nygren and Eric Gremaud and Karl Herbert and Dina Chauhan and Frank Kelly and Crisi Dunster and Joseph Lunec and Marcus Cooke and Mark Evans and Parul Patel and Ian Podmore and Andrea White and Chris Wild and Jo Olliver and Elizabeth Smith",

year = "2002",

month = dec,

day = "1",

doi = "10.1093/carcin/23.12.2129",

language = "English",

volume = "23",

pages = "2129--2133",

journal = "Carcinogenesis",

issn = "0143-3334",

publisher = "Oxford University Press",

number = "12",

}

Collins, A, Gedik, C, Vaughan, N, Wood, S, White, A, Dubois, J, Duez, P, Dehon, G, Rees, JF, Loft, S, Møller, P, Poulsen, H, Riis, B, Weimann, A, Cadet, J, Douki, T, Ravanat, JL, Sauvaigo, S, Faure, H, Morel, I, Morin, B, Epe, B, Phoa, N, Hartwig, A, Pelzer, A, Dolara, P, Casalini, C, Giovannelli, L, Lodovici, M, Olinski, R, Bialkowski, K, Foksinski, M, Gackowski, D, Duračková, Z, Hlinčiková, L, Korytar, P, Sivonová, M, Dušinká, M, Mislanová, C, Viña, J, Lloret, A, Möller, L, Hofer, T, Nygren, J, Gremaud, E, Herbert, K, Chauhan, D, Kelly, F, Dunster, C, Lunec, J, Cooke, M, Evans, M, Patel, P, Podmore, I, White, A, Wild, C, Olliver, J & Smith, E 2002, 'Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus', Carcinogenesis, vol. 23, no. 12, pp. 2129-2133. https://doi.org/10.1093/carcin/23.12.2129

TY - JOUR

T1 - Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories

T2 - An approach to consensus

AU - Collins, Andrew

AU - Gedik, Catherine

AU - Vaughan, Nicholas

AU - Wood, Sharon

AU - White, Ann

AU - Dubois, Jacques

AU - Duez, Pierre

AU - Dehon, Gilles

AU - Rees, Jean François

AU - Loft, Steffen

AU - Møller, Peter

AU - Poulsen, Henrik

AU - Riis, Bente

AU - Weimann, Allan

AU - Cadet, Jean

AU - Douki, Thierry

AU - Ravanat, Jean Luc

AU - Sauvaigo, Sylvie

AU - Faure, Henry

AU - Morel, Isabelle

AU - Morin, Bénédicte

AU - Epe, Bernd

AU - Phoa, Nicole

AU - Hartwig, Andrea

AU - Pelzer, Anke

AU - Dolara, Piero

AU - Casalini, Chiara

AU - Giovannelli, Lisa

AU - Lodovici, Maura

AU - Olinski, Ryszard

AU - Bialkowski, Karol

AU - Foksinski, Marek

AU - Gackowski, Daniel

AU - Duračková, Zdena

AU - Hlinčiková, Lucia

AU - Korytar, Peter

AU - Sivonová, Monika

AU - Dušinká, Mária

AU - Mislanová, Csilla

AU - Viña, José

AU - Lloret, Ana

AU - Möller, Lennart

AU - Hofer, Tim

AU - Nygren, Jonas

AU - Gremaud, Eric

AU - Herbert, Karl

AU - Chauhan, Dina

AU - Kelly, Frank

AU - Dunster, Crisi

AU - Lunec, Joseph

AU - Cooke, Marcus

AU - Evans, Mark

AU - Patel, Parul

AU - Podmore, Ian

AU - White, Andrea

AU - Wild, Chris

AU - Olliver, Jo

AU - Smith, Elizabeth

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 106 guanines, with a median of 10.47 per 106 guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 106 guanines with a median of 5.23 per 106 guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.98 8-oxoGua per 106 guanines, with a median of 0.79 per 106 guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.

AB - The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 106 guanines, with a median of 10.47 per 106 guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 106 guanines with a median of 5.23 per 106 guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.98 8-oxoGua per 106 guanines, with a median of 0.79 per 106 guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.

UR - http://www.scopus.com/inward/record.url?scp=0036955299&partnerID=8YFLogxK

U2 - 10.1093/carcin/23.12.2129

DO - 10.1093/carcin/23.12.2129

M3 - Review article

C2 - 12507938

AN - SCOPUS:0036955299

SN - 0143-3334

VL - 23

SP - 2129

EP - 2133

JO - Carcinogenesis

JF - Carcinogenesis

IS - 12

ER -

Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus

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