Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

Connor J. Dwyer; Colleen A. Cloud; Cindy Wang; Philip Heidt; Paramita Chakraborty; Tara F. Duke; Shannon McGue; Braxton Jeffcoat; Jaclyn Dunne; Logan Johnson; Seungho Choi; Georges J. Nahhas; Amy S. Gandy; Nikolina Babic; Frederick S. Nolte; Philip Howe; Besim Ogretmen; Vamsi K. Gangaraju; Stephen Tomlinson; Brian Madden; Tracy Bridges; Patrick A. Flume; John Wrangle; Mark P. Rubinstein; Prabhakar K. Baliga; Satish N. Nadig; Shikhar Mehrotra

doi:10.1016/j.isci.2021.102489

Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

Connor J. Dwyer
, Colleen A. Cloud
, Cindy Wang
, Philip Heidt
, Paramita Chakraborty
, Tara F. Duke
, Shannon McGue
, Braxton Jeffcoat
, Jaclyn Dunne
, Logan Johnson
, Seungho Choi
, Georges J. Nahhas
, Amy S. Gandy
, Nikolina Babic
, Frederick S. Nolte
, Philip Howe
, Besim Ogretmen
, Vamsi K. Gangaraju
, Stephen Tomlinson
, Brian Madden

Tracy Bridges, Patrick A. Flume, John Wrangle, Mark P. Rubinstein, Prabhakar K. Baliga, Satish N. Nadig, Shikhar Mehrotra

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3% were positive. Of these 3%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.

Original language	English
Article number	102489
Journal	iScience
Volume	24
Issue number	6
DOIs	https://doi.org/10.1016/j.isci.2021.102489
State	Published - 25 Jun 2021
Externally published	Yes

Keywords

Immunology
Infection control in health technology
Virology

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Dwyer, C. J., Cloud, C. A., Wang, C., Heidt, P., Chakraborty, P., Duke, T. F., McGue, S., Jeffcoat, B., Dunne, J., Johnson, L., Choi, S., Nahhas, G. J., Gandy, A. S., Babic, N., Nolte, F. S., Howe, P., Ogretmen, B., Gangaraju, V. K., Tomlinson, S., ... Mehrotra, S. (2021). Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients. iScience, 24(6), Article 102489. https://doi.org/10.1016/j.isci.2021.102489

@article{191394be21894328a362f00af6a0cc0b,

title = "Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients",

abstract = "The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3\% were positive. Of these 3\%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.",

keywords = "Immunology, Infection control in health technology, Virology",

author = "Dwyer, \{Connor J.\} and Cloud, \{Colleen A.\} and Cindy Wang and Philip Heidt and Paramita Chakraborty and Duke, \{Tara F.\} and Shannon McGue and Braxton Jeffcoat and Jaclyn Dunne and Logan Johnson and Seungho Choi and Nahhas, \{Georges J.\} and Gandy, \{Amy S.\} and Nikolina Babic and Nolte, \{Frederick S.\} and Philip Howe and Besim Ogretmen and Gangaraju, \{Vamsi K.\} and Stephen Tomlinson and Brian Madden and Tracy Bridges and Flume, \{Patrick A.\} and John Wrangle and Rubinstein, \{Mark P.\} and Baliga, \{Prabhakar K.\} and Nadig, \{Satish N.\} and Shikhar Mehrotra",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = jun,

day = "25",

doi = "10.1016/j.isci.2021.102489",

language = "English",

volume = "24",

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Dwyer, CJ, Cloud, CA, Wang, C, Heidt, P, Chakraborty, P, Duke, TF, McGue, S, Jeffcoat, B, Dunne, J, Johnson, L, Choi, S, Nahhas, GJ, Gandy, AS, Babic, N, Nolte, FS, Howe, P, Ogretmen, B, Gangaraju, VK, Tomlinson, S, Madden, B, Bridges, T, Flume, PA, Wrangle, J, Rubinstein, MP, Baliga, PK, Nadig, SN & Mehrotra, S 2021, 'Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients', iScience, vol. 24, no. 6, 102489. https://doi.org/10.1016/j.isci.2021.102489

TY - JOUR

T1 - Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

AU - Dwyer, Connor J.

AU - Cloud, Colleen A.

AU - Wang, Cindy

AU - Heidt, Philip

AU - Chakraborty, Paramita

AU - Duke, Tara F.

AU - McGue, Shannon

AU - Jeffcoat, Braxton

AU - Dunne, Jaclyn

AU - Johnson, Logan

AU - Choi, Seungho

AU - Nahhas, Georges J.

AU - Gandy, Amy S.

AU - Babic, Nikolina

AU - Nolte, Frederick S.

AU - Howe, Philip

AU - Ogretmen, Besim

AU - Gangaraju, Vamsi K.

AU - Tomlinson, Stephen

AU - Madden, Brian

AU - Bridges, Tracy

AU - Flume, Patrick A.

AU - Wrangle, John

AU - Rubinstein, Mark P.

AU - Baliga, Prabhakar K.

AU - Nadig, Satish N.

AU - Mehrotra, Shikhar

PY - 2021/6/25

Y1 - 2021/6/25

N2 - The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3% were positive. Of these 3%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.

AB - The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3% were positive. Of these 3%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.

KW - Immunology

KW - Infection control in health technology

KW - Virology

UR - https://www.scopus.com/pages/publications/85106496668

U2 - 10.1016/j.isci.2021.102489

DO - 10.1016/j.isci.2021.102489

M3 - Article

AN - SCOPUS:85106496668

SN - 2589-0042

VL - 24

JO - iScience

JF - iScience

IS - 6

M1 - 102489

ER -

Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

Abstract

Keywords

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