TY - JOUR
T1 - Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin
AU - Scharschmidt, Tiffany C.
AU - Vasquez, Kimberly S.
AU - Pauli, Mariela L.
AU - Leitner, Elizabeth G.
AU - Chu, Kevin
AU - Truong, Hong An
AU - Lowe, Margaret M.
AU - Sanchez Rodriguez, Robert
AU - Ali, Niwa
AU - Laszik, Zoltan G.
AU - Sonnenburg, Justin L.
AU - Millar, Sarah E.
AU - Rosenblum, Michael D.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/4/12
Y1 - 2017/4/12
N2 - Regulatory T cells (Tregs) are required to establish immune tolerance to commensal microbes. Tregs accumulate abruptly in the skin during a defined window of postnatal tissue development. However, the mechanisms mediating Treg migration to neonatal skin are unknown. Here we show that hair follicle (HF) development facilitates the accumulation of Tregs in neonatal skin and that upon skin entry these cells localize to HFs, a primary reservoir for skin commensals. Further, germ-free neonates had reduced skin Tregs indicating that commensal microbes augment Treg accumulation. We identified Ccl20 as a HF-derived, microbiota-dependent chemokine and found its receptor, Ccr6, to be preferentially expressed by Tregs in neonatal skin. The Ccl20-Ccr6 pathway mediated Treg migration in vitro and in vivo. Thus, HF morphogenesis, commensal microbe colonization, and local chemokine production work in concert to recruit Tregs into neonatal skin, thereby establishing this tissue Treg niche early in life.
AB - Regulatory T cells (Tregs) are required to establish immune tolerance to commensal microbes. Tregs accumulate abruptly in the skin during a defined window of postnatal tissue development. However, the mechanisms mediating Treg migration to neonatal skin are unknown. Here we show that hair follicle (HF) development facilitates the accumulation of Tregs in neonatal skin and that upon skin entry these cells localize to HFs, a primary reservoir for skin commensals. Further, germ-free neonates had reduced skin Tregs indicating that commensal microbes augment Treg accumulation. We identified Ccl20 as a HF-derived, microbiota-dependent chemokine and found its receptor, Ccr6, to be preferentially expressed by Tregs in neonatal skin. The Ccl20-Ccr6 pathway mediated Treg migration in vitro and in vivo. Thus, HF morphogenesis, commensal microbe colonization, and local chemokine production work in concert to recruit Tregs into neonatal skin, thereby establishing this tissue Treg niche early in life.
KW - commensal-specific immune tolerance
KW - hair follicles
KW - migration
KW - regulatory T cells
KW - skin commensal microbes
KW - tissue development
UR - http://www.scopus.com/inward/record.url?scp=85016057605&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2017.03.001
DO - 10.1016/j.chom.2017.03.001
M3 - Article
C2 - 28343820
AN - SCOPUS:85016057605
SN - 1931-3128
VL - 21
SP - 467-477.e5
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -