Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin

Tiffany C. Scharschmidt, Kimberly S. Vasquez, Mariela L. Pauli, Elizabeth G. Leitner, Kevin Chu, Hong An Truong, Margaret M. Lowe, Robert Sanchez Rodriguez, Niwa Ali, Zoltan G. Laszik, Justin L. Sonnenburg, Sarah E. Millar, Michael D. Rosenblum

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

Regulatory T cells (Tregs) are required to establish immune tolerance to commensal microbes. Tregs accumulate abruptly in the skin during a defined window of postnatal tissue development. However, the mechanisms mediating Treg migration to neonatal skin are unknown. Here we show that hair follicle (HF) development facilitates the accumulation of Tregs in neonatal skin and that upon skin entry these cells localize to HFs, a primary reservoir for skin commensals. Further, germ-free neonates had reduced skin Tregs indicating that commensal microbes augment Treg accumulation. We identified Ccl20 as a HF-derived, microbiota-dependent chemokine and found its receptor, Ccr6, to be preferentially expressed by Tregs in neonatal skin. The Ccl20-Ccr6 pathway mediated Treg migration in vitro and in vivo. Thus, HF morphogenesis, commensal microbe colonization, and local chemokine production work in concert to recruit Tregs into neonatal skin, thereby establishing this tissue Treg niche early in life.

Original languageEnglish
Pages (from-to)467-477.e5
JournalCell Host and Microbe
Volume21
Issue number4
DOIs
StatePublished - 12 Apr 2017
Externally publishedYes

Keywords

  • commensal-specific immune tolerance
  • hair follicles
  • migration
  • regulatory T cells
  • skin commensal microbes
  • tissue development

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