Combinatorial array-based enzymatic polyester synthesis

Dae Yun Kim, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


A combinatorial strategy for biocatalytic polymer synthesis is demonstrated. A library of polymers was synthesized in 96 deep-well plates using AA-BB polycondensations of acyl donors and acceptors. The library was based on four straight-chain diesters as acyl donors (C3-C10) with aliphatic/aromatic diols as well as more diverse structures including carbohydrates, nucleic acids, and a natural steroid diol used as acyl acceptors. The lipase from Candida antarctica was active in acetonitrile and was capable of catalyzing the polycondensation of the aforementioned monomers to polymers with Mw's reaching as high as 20,000 Da, including the preparation of novel sugar-containing polyesters. The combinatorial approach to biocatalytic polymer synthesis described herein serves as a foundation for polymeric materials discovery by demonstrating that polymer arrays can be produced from structurally complex monomers.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalBiotechnology and Bioengineering
Issue number3
StatePublished - 2001
Externally publishedYes


  • Combinatorial chemistry
  • Combinatorial synthesis
  • Enzymatic polymerization
  • Enzyme
  • Lipases
  • Polyesters


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