Colonization of the live biotherapeutic product VE303 and modulation of the microbiota and metabolites in healthy volunteers

Melissa Dsouza, Rajita Menon, Emily Crossette, Shakti K. Bhattarai, Jessica Schneider, Yun Gi Kim, Shilpa Reddy, Silvia Caballero, Cintia Felix, Louis Cornacchione, Jared Hendrickson, Andrea R. Watson, Samuel S. Minot, Nick Greenfield, Lisa Schopf, Rose Szabady, Juan Patarroyo, William Smith, Pratibha Harrison, Ed J. KuijperCiaran P. Kelly, Bernat Olle, Dmitri Bobilev, Jeffrey L. Silber, Vanni Bucci, Bruce Roberts, Jeremiah Faith, Jason M. Norman

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Manipulation of the gut microbiota via fecal microbiota transplantation (FMT) has shown clinical promise in diseases such as recurrent Clostridioides difficile infection (rCDI). However, the variable nature of this approach makes it challenging to describe the relationship between fecal strain colonization, corresponding microbiota changes, and clinical efficacy. Live biotherapeutic products (LBPs) consisting of defined consortia of clonal bacterial isolates have been proposed as an alternative therapeutic class because of their promising preclinical results and safety profile. We describe VE303, an LBP comprising 8 commensal Clostridia strains under development for rCDI, and its early clinical development in healthy volunteers (HVs). In a phase 1a/b study in HVs, VE303 is determined to be safe and well-tolerated at all doses tested. VE303 strains optimally colonize HVs if dosed over multiple days after vancomycin pretreatment. VE303 promotes the establishment of a microbiota community known to provide colonization resistance.

Original languageEnglish
Pages (from-to)583-598.e8
JournalCell Host and Microbe
Issue number4
StatePublished - 13 Apr 2022


  • C. difficile infection
  • antibiotics
  • live biotherapeutic product
  • metabolites
  • metagenomics
  • microbiome
  • pharmacology


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