Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro; A. Bertolotto; I. Germano; G. Giaccone; M. T. Giordana; A. Migheli; D. Schiffer

doi:10.1007/BF00679991

Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro, A. Bertolotto, I. Germano, G. Giaccone, M. T. Giordana, A. Migheli, D. Schiffer

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Abstract

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

Original language	English
Pages (from-to)	157-163
Number of pages	7
Journal	Histochemistry
Volume	80
Issue number	2
DOIs	https://doi.org/10.1007/BF00679991
State	Published - Mar 1984
Externally published	Yes

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title = "Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation",

abstract = "The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.",

author = "A. Mauro and A. Bertolotto and I. Germano and G. Giaccone and Giordana, \{M. T.\} and A. Migheli and D. Schiffer",

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AU - Mauro, A.

AU - Bertolotto, A.

AU - Germano, I.

AU - Giaccone, G.

AU - Giordana, M. T.

AU - Migheli, A.

AU - Schiffer, D.

PY - 1984/3

Y1 - 1984/3

N2 - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

AB - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

UR - https://www.scopus.com/pages/publications/0021235758

U2 - 10.1007/BF00679991

DO - 10.1007/BF00679991

M3 - Article

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AN - SCOPUS:0021235758

SN - 0301-5564

VL - 80

SP - 157

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JO - Histochemistry

JF - Histochemistry

IS - 2

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Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

Abstract

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