TY - JOUR
T1 - Cohesin SMC1β is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination
AU - Revenkova, Ekaterina
AU - Eijpe, Maureen
AU - Heyting, Christa
AU - Hodges, Craig A.
AU - Hunt, Patricia A.
AU - Liebe, Bodo
AU - Scherthan, Harry
AU - Jessberger, Rolf
N1 - Funding Information:
We acknowledge the support of the Mount Sinai Mouse Genetics Shared Research Facility (K. Kelley), funded by a grant from the NIH/NCI (R24CA88302). We thank A. Pastink, J.-M. Peters, T. de Lange, P. de Boer, P. Cohen, P. Moens and T. Ashley for generous gifts of antibodies; A. Firooznia for the preparation of recombinant STAG3 antigen and anti-STAG3 antibody; and E. de Boer for the preparation of recombinant MLH1 antigen and anti-MLH1 antibody. This work was supported by a grant from the NIH (GM62517) to R.J., a grant from the DFG (Sche350/8-4) to H.S., a grant from the NIH (HD37502) to P.A.H., and a grant from the EU (contract no. QLK3-2000-00365) to C.H.
PY - 2004/6
Y1 - 2004/6
N2 - Sister chromatid cohesion ensures the faithful segregation of chromosomes in mitosis and in both meiotic divisions. Meiosis-specific components of the cohesin complex, including the recently described SMC1 isoform SMC1β, were suggested to be required for meiotic sister chromatid cohesion and DNA recombination. Here we show that SMC1β-deficient mice of both sexes are sterile. Male meiosis is blocked in pachytene; female meiosis is highly error-prone but continues until metaphase II. Prophase axial elements (AEs) are markedly shortened, chromatin extends further from the AEs, chromosome synapsis is incomplete, and sister chromatid cohesion in chromosome arms and at centromeres is lost prematurely. In addition, crossover-associated recombination foci are absent or reduced, and meiosis-specific perinuclear telomere arrangements are impaired. Thus, SMC1β has a key role in meiotic cohesion, the assembly of AEs, synapsis, recombination, and chromosome movements.
AB - Sister chromatid cohesion ensures the faithful segregation of chromosomes in mitosis and in both meiotic divisions. Meiosis-specific components of the cohesin complex, including the recently described SMC1 isoform SMC1β, were suggested to be required for meiotic sister chromatid cohesion and DNA recombination. Here we show that SMC1β-deficient mice of both sexes are sterile. Male meiosis is blocked in pachytene; female meiosis is highly error-prone but continues until metaphase II. Prophase axial elements (AEs) are markedly shortened, chromatin extends further from the AEs, chromosome synapsis is incomplete, and sister chromatid cohesion in chromosome arms and at centromeres is lost prematurely. In addition, crossover-associated recombination foci are absent or reduced, and meiosis-specific perinuclear telomere arrangements are impaired. Thus, SMC1β has a key role in meiotic cohesion, the assembly of AEs, synapsis, recombination, and chromosome movements.
UR - https://www.scopus.com/pages/publications/2942729457
U2 - 10.1038/ncb1135
DO - 10.1038/ncb1135
M3 - Article
C2 - 15146193
AN - SCOPUS:2942729457
SN - 1465-7392
VL - 6
SP - 555
EP - 562
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 6
ER -